Pedosphere 32(5): 673--685, 2022
ISSN 1002-0160/CN 32-1315/P
©2022 Soil Science Society of China
Published by Elsevier B.V. and Science Press
A highly effective polycyclic aromatic hydrocarbon-degrading bacterium, Paracoccus sp. HPD-2, shows opposite remediation potential in two soil types
Wei CHEN2, Ying TENG1, Wenjie REN1, Yongming LUO1, Yao YU3
1Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210018 (China)
2Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014 (China)
3School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009 (China)
      Bioaugmentation is an efficient and eco-friendly strategy for the bioremediation of polycyclic aromatic hydrocarbons (PAHs). Since the degrading abilities of soils can greatly alter the abilities of PAH-degrading bacteria, illustrating the potential and mechanism of highly efficient degrading bacteria in different soil environments is of great importance for bioremediation. A PAH-degrading bacterium, Paracoccus aminovorans HPD-2, and two soil types, red and paddy soils, with distinct PAH-degrading abilities, were selected for this study. A soil microcosm experiment was performed by adding pyrene (PYR) and benzo[a]pyrene (B[a]P). Illumina sequencing was used to examine bacterial community structure. The results showed that inoculation with HPD-2 significantly elevated PYR and B[a]P degradation rates by 44.7% and 30.7%, respectively, in the red soil, while it only improved the degradation rates by 1.9% and 11%, respectively, in the paddy soil. To investigate the underlying mechanism, the fate of strain HPD-2 and the response of the indigenous bacterial communities were determined. Strain HPD-2 occupied certain niches in both soils, and the addition of the bacterium changed the native community structure more noticeably in the red soil than in the paddy soil. The addition of PAHs and strain HPD-2 significantly changed the abundances of 7 phyla among the 15 detected phyla in the red soil. In the paddy soil, 5 of the 12 dominant phyla were significantly affected by PAHs and the inoculation of HPD-2, while 6 new phyla were detected in the low-abundance phyla (< 0.1%). The abundances of Massilia, Burkholderia, and Rhodococcus genera with PAH degradation efficiency were significantly increased by the inoculation of HPD-2 in the red soil during 42 d of incubation. Meanwhile, in the paddy soil, the most dominant effective genus, Massilia, was reduced by HPD-2 inoculation. This research revealed the remediation ability and inherent mechanism of the highly effective PAH-degrading strain HPD-2 in two different soil types, which would provide a theoretical basis for the application of degrading bacteria in different soils.
Key Words:  bacterial inoculation,benzo[a]pyrene,bioremediation,organic contaminant,paddy soil,Paracoccus aminovorans,pyrene,red soil
Citation: Chen W, Teng Y, Ren W J, Luo Y M, Yu Y. 2022. A highly effective polycyclic aromatic hydrocarbon-degrading bacterium, Paracoccus sp. HPD-2, shows opposite remediation potential in two soil types. Pedosphere. 32(5): 673-685.
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