Pedosphere 32(4): 532--542, 2022
ISSN 1002-0160/CN 32-1315/P
©2022 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Inhibition of ammonia-oxidizing bacteria promotes the growth of ammonia-oxidizing archaea in ammonium-rich alkaline soils
Chang YIN1, Xiaoping FAN1, Hao CHEN1, Mujun YE1, Guochao YAN1, Tingqiang LI1, Hongyun PENG1, Shengzhe E2, Zongxian CHE2, Steven A. WAKELIN3, Yongchao LIANG1
1Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou 310058 (China)
2Institute of Soil and Fertilizer and Water-Saving Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou 730070 (China)
3Scion Research, P.O. Box 29237, Christchurch 8011 (New Zealand)
      Disparities in the substrate affinity and tolerance threshold for ammonia have been believed to play a key role in driving niche differentiation between ammonia-oxidizing archaea (AOA) and bacteria (AOB); however, recent surveys argue that direct competition between AOA and AOB is also important in this phenomenon. Accordingly, it is reasonable to predict that diverse AOA lineages would grow in ammonium (NH4+)-rich alkaline arable soils if AOB growth is suppressed. To test this hypothesis, a microcosm study was established using three different types of alkaline arable soils, in which a high NH4+ concentration (200 μg N g-1 dry soil) was maintained by routinely replenishing urea and the activities of AOB were selectively inhibited by 1-octyne or 3,4-dimethylpyrazole phosphate (DMPP). Compared with amendment with urea alone, 1-octyne partially retarded AOB growth, while DMPP completely inhibited AOB. Both inhibitors accelerated the growth of AOA, with significantly higher ratios of abundance of AOA to AOB observed with DMPP amendment across soils. Nonmetric multidimensional scaling analysis (NMDS) indicated that different treatments significantly altered the community structures of both AOA and AOB and AOA OTUs enriched by high-NH4+ amendment were taxonomically constrained across the soils tested and closely related to Nitrososphaera viennensis EN76 and N. garnensis. Given that these representative strains have been demonstrated to be sensitive to high ammonia concentrations, our results suggest that it is the competitiveness for ammonia, rather than disparities in substrate affinity and tolerance threshold for ammonia, that drives niche differentiation between these phylotypes and AOB in NH4+-rich alkaline soils.
Key Words:  community structure,3,4-dimethylpyrazole phosphate,niche differentiation,1-octyne,substrate affinity,tolerance threshold
Citation: Yin C, Fan X P, Chen H, Ye M J, Yan G C, Li T Q, Peng H Y, E S Z, Che Z X, Wakelin S A, Liang Y C. 2022. Inhibition of ammonia-oxidizing bacteria promotes the growth of ammonia-oxidizing archaea in ammonium-rich alkaline soils. Pedosphere. 32(4): 532-542.
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