Pedosphere 31(4): 538--548, 2021
ISSN 1002-0160/CN 32-1315/P
©2021 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Effect of long-term fertilization on bacterial communities in wheat endosphere
Yuying MA1,2, Pamela WEISENHORN3, Xisheng GUO4, Daozhong WANG4, Teng YANG2, Yu SHI2, Huanchao ZHANG1, Haiyan CHU2,5
1Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037 (China)
2State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)
3Biosciences Division, Argonne National Laboratory, Argonne, IL 60439 (USA)
4Key Laboratory of Nutrient Cycling and Resources Environment of Anhui Province, Soil and Fertilizer Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031 (China)
5University of Chinese Academy of Sciences, Beijing 100049 (China)
ABSTRACT
      Fertilization has been shown to exert a significant influence on soil microorganisms and directly and indirectly influences plant growth and survival in agroecosystems. However, it is unknown whether fertilization affects endophytic microbial communities, which are ubiquitous and intimately associated with plant growth and health. Herein, we investigated endophytic bacterial communities in wheat leaves and roots under different long-term fertilization regimes, including NPK chemical fertilizer and NPK chemical fertilizer combined with wheat straw, pig manure, or cow manure. Endophytic bacterial community composition considerably differed in leaves and roots. Although different fertilization treatments did not affect the endophytic bacterial species richness or phylogenetic diversity in either leaves or roots, the community composition was significantly altered, particularly in roots. The endophytic bacterial co-occurrence network in leaves was more complex and stable than that in roots. Furthermore, many of the keystone species that were identified by their topological positions in the co-occurrence networks of leaves and roots were involved in plant growth and fitness. The total relative abundance of keystone species was the highest in the NPK plus cow manure treatment in both leaves and roots. Overall, our results suggest that different fertilization regimes can strongly affect endophytic bacterial communities, and the combination of NPK fertilizer and cow manure promoted the relative abundance of the key endophytic bacterial microbiota in both leaves and roots, which might be beneficial for plants in agroecosystems.
Key Words:  co-occurrence network,endophytic bacterial community,key microbiota,long-term fertilization,organic matter
Citation: Ma Y Y, Weisenhorn P, Guo X S, Wang D Z, Yang T, Shi Y, Zhang H C, Chu H Y. 2021. Effect of long-term fertilization on bacterial communities in wheat endosphere. Pedosphere. 31(4): 538-548.
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