Pedosphere 30(5): 684--693, 2020
ISSN 1002-0160/CN 32-1315/P
©2020 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Halophile plant growth-promoting rhizobacteria induce salt tolerance traits in wheat seedlings (Triticum aestivum L.)
Maryam SAFDARIAN1, Hossein ASKARI2, Ghorbanali NEMATZADEH1, Adriano SOFO3
1Sari Agricultural Sciences and Natural Resources University, Mazandaran Province, Sari 4818168984 (Iran)
2Department of Biotechnology, Faculty of New Technologies and Energy Engineering, Shahid Beheshti University, Tehran 1983969411 (Iran)
3Department of European and Mediterranean Cultures:Architecture, Environment, Cultural Heritage (DiCEM), Università degli Studi della Basilicata, Via Lanera 20, Matera 75100 (Italy)
      Salinity is one of the most important growth-limiting factors for most crops in arid and semi-arid regions; however, the use of plant growth-promoting rhizobacteria isolated from saline soils could reduce the effects of saline stress in crops. This study aimed to evaluate the efficiency of plant growth-promoting rhizobacteria (PGPRs), isolated from the rhizosphere of halophile plants, for the growth, Na+/K+ balance, ethylene emission, and gene expression of wheat seedlings (Triticum aestivum L.) grown under saline conditions (100 mmol L-1 NaCl) for 14 d. A total of 118 isolates obtained from saline soils of the deserts of Iran were tested for their capacity as PGPRs. Out of the 118 isolates, 17 could solubilize phosphate (Ca3(PO4)2), 5 could produce siderophores, and 16 could synthesize indole-3-acetic acid. Additionally, PGPRs were also evaluated for aminocyclopropane-1-carboxylate deaminase activity. A pot experiment was conducted to evaluate the ability of 28 PGPR isolates to promote growth, regulate Na+/K+ balance, and decrease ethylene emissions in plants. The most efficient PGPRs were Arthrobacter aurescens, Bacillus atrophaeus, Enterobacter asburiae, and Pseudomonas fluorescens. Gene expression analysis revealed the up-regulation of H+-PPase, HKT1, NHX7, CAT, and APX expression in roots of Enterobacter-inoculated salt-stressed plants. Salt-tolerant rhizobacteria exhibiting plant growth-promoting traits can facilitate the growth of wheat plants under saline conditions. Our results indicate that the isolation of these bacteria may be useful for formulating new inoculants to improve wheat cropping systems in saline soils.
Key Words:  ethylene emission,gene expression,growth-promoting trait,indole-3-acetic acid,phosphate solubility,saline soil,siderophore
Citation: Safdarian M, Askari H, Nematzadeh G, Sofo A. 2020. Halophile plant growth-promoting rhizobacteria induce salt tolerance traits in wheat seedlings (Triticum aestivum L.). Pedosphere. 30(5):684-693.
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