Pedosphere 30(5): 618--627, 2020
ISSN 1002-0160/CN 32-1315/P
©2020 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Optimization and modeling of glyphosate biodegradation by a novel Comamonas odontotermitis P2 through response surface methodology
Sadiqa FIRDOUS1,2, Samina IQBAL1,2, Samina ANWAR1
1Soil and Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad 38000 (Pakistan)
2Pakistan Institute of Engineering and Applied Sciences (PIEAS), Nilore, Islamabad 45650 (Pakistan)
      Glyphosate is an important organophosphonate herbicide used to eliminate grasses and herbaceous plants in many vegetation management situations. Its extensive use is causing environmental pollution, and consequently, there is a need to remove it from the environment using an eco-friendly and cost-effective method. As a step to address this problem, a novel bacterial strain Comamonas odontotermitis P2, capable to utilize glyphosate as a carbon (C) and/or phosphorus (P) source, was isolated from a glyphostate-contaminated field soil in Australia and characterized. Response surface methodology (RSM) employing a 23 full factorial central composite design was used to optimize glyphosate degradation by C. odontotermitis P2 under various culture conditions. The strain C. odontotermitis P2 was proficient in degrading 1.5 g L-1 glyphosate completely within 104 h. The optimal conditions for the degradation of glyphosate were found to be pH 7.4, 29.9℃, and an inoculum density of 0.54 g L-1, resulting in a maximum degradation of 90%. Sequencing of glyphosate oxidoreductase (GOX) and C-P lyase (phnJ) genes from C. odontotermitis P2 revealed 99% and 93% identities to already reported bacterial GOX and phnJ genes, respectively. The presence of these two genes in C. odontotermitis indicates its potential to degrade glyphosate through GOX and C-P lyase metabolic pathways. This study demonstrates the potential of C. odontotermitis P2 for efficient degradation of glyphosate, which can be exploited for remediation of glyphosate.
Key Words:  bacterial strain,central composite rotatable design,glyphosate oxidoreductase,high-performance liquid chromatography,inoculum density,organophosphonate herbicide,remediation
Citation: Firdous S, Iqbal S, Anwar S. 2020. Optimization and modeling of glyphosate biodegradation by a novel Comamonas odontotermitis P2 through response surface methodology. Pedosphere. 30(5):618-627.
View Full Text

Copyright © 2023 Editorial Committee of PEDOSPHERE. All rights reserved.
Address: P. O. Box 821, 71 East Beijing Road, Nanjing 210008, China    E-mail:
Technical support: Beijing E-Tiller Co.,Ltd.