Pedosphere 30(4): 487--495, 2020
ISSN 1002-0160/CN 32-1315/P
©2020 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Impact of monoculture of poplar on rhizosphere microbial communities over time
Qian LU, Jinchi ZHANG, Lisha CHEN
Department of Water and Soil Conservation, College of Forestry, Nanjing Forestry University, Nanjing 210037(China)
ABSTRACT
      To date, research on the adverse effects of continuous cropping has focused on field crops; forest plantations, despite their very significant economic and ecological value, have received less attention. The evolution of microbial community in the rhizosphere of forest plantations, in particular, has rarely been examined. In this study, changes in the size, composition, and structure of bacterial and fungal communities in the rhizosphere of different generations of poplar (Populus deltoides) plantations were studied using real-time polymerase chain reaction (PCR) and PCR-denaturing gradient gel electrophoresis and compared with the composition of the microbial community in a bare land (control) adjacent to the plantation sites through sequencing analysis and by constructing phylogenetic trees. The numbers of bacteria and fungi increased significantly with successive poplar generations, although the increases in the two groups were not parallel. When compared with the control, the bacterial community increased greatly in the second generation, and the most significant increase occurred in the third generation. In contrast, the most significant increase in the fungal community occurred in the first generation, and the increase in the third generation was insignificant. In terms of community composition, the first generation showed little change in either community; however, the second generation showed remarkable changes in the bacterial community, and the third in the fungal community. The numbers of Gammaproteobacteria, Alphaproteobacteria, and Actinobacteria increased by 10.92%, 7.38%, and 5.46%, respectively, whereas those of Acidobacteria decreased by 18.38% in the second generation. These changes in the number and composition of microbial communities in the rhizosphere could be one of the reasons for the decline in yield and quality associated with long-term monoculture.
Key Words:  bacterial community,continuous cropping,denaturing gradient gel electrophoresis,forest plantations,fungal community,polymerase chain reaction,Populus deltoides
Citation: Lu Q, Zhang J C, Chen L S. 2020. Impact of monoculture of poplar on rhizosphere microbial communities over time. Pedosphere. 30(4): 487–495.
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