Pedosphere 30(3): 426--432, 2020
ISSN 1002-0160/CN 32-1315/P
©2020 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Root cell walls and phytochelatins in low-cadmium cultivar of Brassica parachinensis
Yan SUN1, Hui YE2, Zebin WEI1, Xuhui KONG3, Qitang WU1
1Key Laboratory on Soil Environment and Waste Reuse in Agriculture of Guangdong Higher Education Institutes, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642 (China)
2College of Life Science, South China Agricultural University, Guangzhou 510642 (China)
3Key Laboratory on Agricultural Ecology and Rural Environment of Guangdong Higher Education Institutes, South China Agricultural University, Guangzhou 510642 (China)
ABSTRACT
      ‘Lvbao-701’ is a cultivar of Chinese flowering cabbage (Brassica parachinensis) that exhibits low cadmium (Cd) accumulation and high Cd tolerance. In this study, this cultivar was compared with a high-Cd accumulating cultivar, ‘Chixin-4’, to characterize the mechanisms influencing Cd accumulation in B. parachinensis. Root cell walls were isolated by dissolving the cytoplasm with an organic solvent, and root Cd and phytochelatin (PC) contents were analyzed. In addition, a PC synthase gene fragment was cloned and expressed under Cd stress conditions. The proportions of Cd bound to root cell walls were higher in the ‘Lvbao-701’ plants (68.32%-76.80%) than in the ‘Chixin-4’ plants (35.36%-54.18%) after exposure to Cd stress. The proportions of Cd bound to root cell walls measured using cell walls isolated with a non-destructive method were higher than those obtained using a conventional method that required grinding and centrifugation. Exposure to Cd stress induced the PC production and resulted in higher PC contents in the ‘Lvbao-701’ plants than in the ‘Chixin-4’ plants. Cloning and expression analysis of a B. parachinensis PC synthase cDNA fragment indicated that PC synthase gene expression was induced by Cd and occurred mainly in the roots of both ‘Lvbao-701’ and ‘Chixin-4’ plants. However, the PC synthase gene expression level was higher in the ‘Lvbao-701’ roots than in the ‘Chixin-4’ roots. Therefore, a higher abundance of Cd in the root cell walls of ‘Lvbao-701’ and up-regulated PC production in the roots are probably the main reasons why ‘Lvbao-701’ exhibits lower Cd translocation to the shoots and higher tolerance to Cd than ‘Chixin-4’.
Key Words:  cadmium accumulation,cadmium stress,cadmium tolerance,cadmium translocation,gene expression,Lvbao-701,phytochelatin synthase
Citation: Sun Y, Ye H, Wei Z B, Kong X H, Wu Q T. 2020. Root cell walls and phytochelatins in low-cadmium cultivar of Brassica parachinensis. Pedosphere. 30(3): 426-432.
View Full Text



Copyright © 2024 Editorial Committee of PEDOSPHERE. All rights reserved.
Address: No.298 Chuangyou Road, Jiangning District, Nanjing 210008, China PostCode:211135    E-mail: pedosphere@issas.ac.cn
Technical support: Beijing E-Tiller Co.,Ltd.