Pedosphere 30(3): 343--351, 2020
ISSN 1002-0160/CN 32-1315/P
©2020 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Anammox bacterial abundance and biodiversity in greenhouse vegetable soil are influenced by high nitrate content
Hongyi QIN1,3, Huan DENG2,3, Cheng HAN1,3, Wenhui ZHONG1,3
1School of Geography Science, Nanjing Normal University, Nanjing 210023 (China)
2School of Environment, Nanjing Normal University, Nanjing 210023 (China)
3Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing 210023 (China)
      The anaerobic ammonium oxidizing (anammox) process has been found to play an important role in terrestrial ecosystems in recent years. However, the diversity and abundance of anammox bacteria in nitrogen (N)-rich agricultural soils under high fertilizer greenhouse conditions are still unclear. Two greenhouse fields with different N fertilizer input levels were chosen, and their soil profiles were studied with molecular technologies, including quantitative polymerase chain reaction assay, a clone library, and phylogenetic analysis based on hzsB (encoding anammox hydrazine synthase β-subunit) gene. Molecular analyses suggested that anammox bacteria were at their highest density at 10-20 cm soil depth, and that the anammox bacterial abundance was significantly lower at high N than at low N. Candidatus Brocadia was the sole anammox bacterial genus throughout the soil depth profiles. The highest diversity of anammox bacteria was found at 30-40 cm soil depth, and different phylotypic clusters of Candidatus Brocadia were associated with specific soil environmental factors, such as nitrates, soil depth, and total N. Correlation analyses and redundancy analyses confirmed that high nitrate content associated with high N fertilizer input had a significant negative influence on the abundance and biodiversity of anammox bacteria. These results imply that excessive use of N fertilizer would affect arid land soil N loss to the atmosphere by the anammox pathway.
Key Words:  anammox pathway,community structure,fertilizer input level,greenhouse soil,hydrazine gene,nitrogen fertilizer,nitrogen pollution,soil degradation
Citation: Qin H Y, Deng H, Han C, Zhong W H. 2020. Anammox bacterial abundance and biodiversity in greenhouse vegetable soil are influenced by high nitrate content. Pedosphere. 30(3): 343-351.
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