Pedosphere 30(2): 285--292, 2020
ISSN 1002-0160/CN 32-1315/P
©2020 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Effect of environmental C/N ratio on activities of lignin-degrading enzymes produced by Phanerochaete chrysosporium
Saihua HUANG3, Deyin HUANG3, Qitang WU1, Meifang HOU2,3, Xiaoyan TANG2,3, Jian ZHOU2
1College of Natural Resources and Environment, South China Agricultural University, 483 Wushan Street, Guangzhou 510642 (China)
2School of Ecological Technology and Engineering, Shanghai Institute of Technology, 100 Haiquan, Shanghai 201418 (China)
3Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-environmental and Soil Sciences, 808 Tianyuan Road, Guangzhou 510650 (China)
Corresponding Author:Saihua HUANG
ABSTRACT
      Lignin-degrading enzymes secreted by white rot fungi play an important role in the degradation of lignin and persistent organic pollutants (POPs). In this study, effect of environmental C/N ratio on the activities of lignin-degrading enzymes, lignin peroxide (LiP) and manganese peroxidase (MnP), produced by Phanerochaete chrysosporium, a white rot fungus, was investigated. Glucose was used as C source, and ammonium tartrate of different concentrations was used as N source to provide different C/N ratios. Relationships between LiP and MnP activities and environmental C/N ratio were explored. The results showed that the higher the N source concentration, the faster the mycelium pellets aged. The faster the mycelium dry weight increased, the higher the LiP and MnP activities. A high C/N ratio was a necessary condition for the secretion of LiP or MnP. In addition, mycelium dry weight essentially affected enzyme activities. In the 122 C/N ratio and 50 C/N ratio treatments, mycelium dry weight essentially affected MnP activity and both LiP and MnP activities, respectively.
Key Words:  biodegration,lignin peroxide,manganese peroxidase,peroxidase,persistent organic pollutant,polysaccharide,white rot fungus
Citation: Huang S H, Huang D Y, Wu Q T, Hou M F, Tang X Y, Zhou J. 2020. Effect of environmental C/N ratio on activities of lignin-degrading enzymes produced by Phanerochaete chrysosporium. Pedosphere. 30(2): 285-292.
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