Pedosphere 30(1): 73--86, 2020
ISSN 1002-0160/CN 32-1315/P
©2020 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Chronic effects of different fertilization regimes on nirS-type denitrifier communities across the black soil region of Northeast China
Xiaojing HU1, Junjie LIU1, Dan WEI2,5, Ping ZHU3, Xi'an CUI4, Baoku ZHOU2, Xueli CHEN2, Jian JIN1, Xiaobing LIU1, Guanghua WANG1
1Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081(China)
2Institute of Soil and Fertilizer and Environment Resources, Heilongjiang Academy of Agricultural Sciences, Harbin 150086(China)
3Institute of Agricultural Resource and Environment, Jilin Academy of Agricultural Sciences, Changchun 130033(China)
4Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300(China)
5Institute of Plant Nutrition and Resources, Beijing Academy of Agricultureand Forestry Sciences, Beijing 100097(China)
ABSTRACT
      Denitrification is one of the major processes causing nitrogen loss from arable soils. This study aimed to investigate the responses of nirS-type denitrifier communities to different chronic fertilization regimes across the black soil region of Northeast China. Soil samples were collected from sites located in the north (NB), middle (MB), and south (SB) of the black soil region of Northeast China, each with four chronic fertilization regimes:no fertilizer (NoF), chemical fertilizer (CF), manure (M), and chemical fertilizer plus manure (CFM). Methods of quantitative polymerase chain reaction (qPCR) and Illumina MiSeq sequencing were applied to assess the abundance and composition of denitrifier communities by targeting the nirS gene. The results showed that the M and CFM regimes significantly increased the abundances of nirS-type denitrifiers compared with NoF at the three locations. The majority of nirS sequences were grouped as unclassified denitrifiers, and the different fertilizers induced little variation in the relative abundance of known nirS-type denitrifier taxa. Over 90% of the sequences were shared among the four fertilization regimes at each location, but none of the abundant operational taxonomic units (OTUs) were shared among the three locations. Principal coordinate analysis (PCoA) revealed that the communities of nirS-type denitrifier were separated into three groups that corresponded with their locations. Although similar fertilization regimes did not induce consistent changes in the nirS-type denitrifier communities, soil pH and NO3- -N content simultaneously and significantly influenced the structure of nirS-type denitrifier communities at the three locations. Our results highlight that geographical separation rather than chronic fertilization was the dominant factor determining the nirS-type denitrifier community structures, and similar chronic fertilization regimes did not induce consistent shifts of nirS-type denitrifier communities in the black soils.
Key Words:  denitrification,denitrifier diversity,denitrifying gene,geographical separation,Illumina MiSeq sequencing,manure,Mollisols,nirS gene,quantitative polymerase chain reaction
Citation: Hu X J, Liu J J, Wei D, Zhu P, Cui X A, Zhou B K, Chen X L, Jin J, Liu X B, Wang G H. 2020. Chronic effects of different fertilization regimes on nirS-type denitrifier communities across the black soil region of Northeast China. Pedosphere. 30(1):73-86.
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