Pedosphere 29(5): 656--664, 2019
ISSN 1002-0160/CN 32-1315/P
©2019 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Distribution of Manganese(II) Chemical Forms on Soybean Roots and Manganese(II) Toxicity
LIU Yuan1,2,3, LI Zhongyi1, XU Renkou1
1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008(China)
2Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, Xinxiang 453002(China)
3Key Laboratory of High-Efficient and Safe Utilization of Agriculture Water Resources, Chinese Academy of Agricultural Sciences, Xinxiang 453002(China)
ABSTRACT
      Distribution of chemical forms of manganese(Ⅱ) (Mn(Ⅱ)) on plant roots may affect Mn(Ⅱ) absorption by plants and toxicity of Mn(Ⅱ) to plants at its high level. The chemical forms of Mn(Ⅱ) on soybean roots were investigated to determine the main factors that affect their distribution and relationship with Mn(Ⅱ) plant toxicity. Fresh soybean roots were reacted with Mn(Ⅱ) in solutions, and Mn(Ⅱ) adsorbed on the roots was differentiated into exchangeable, complexed, and precipitated forms through sequential extraction with KNO3, EDTA, and HCl. The exchangeable Mn(Ⅱ) content on the roots was the highest, followed by the complexed and precipitated Mn(Ⅱ) contents. Mn(Ⅱ) toxicity to the roots was greater at pH 5.5 than at pH 4.2 due to the larger amount of exchangeable Mn(Ⅱ) at higher pH. The cations Al3+, La3+, Ca2+, Mg2+, and NH4+ competed with Mn(Ⅱ) for cation exchange sites on the root surfaces and thus reduced exchangeable Mn(Ⅱ) on the roots, in the order Al3+, La3+ > Ca2+, Mg2+ > NH4+. Al3+ and La3+ at 100 μmol L-1 decreased exchangeable Mn(Ⅱ) by 80% and 79%, respectively, and Ca2+ and Mg2+ at 1 mmol L-1 decreased exchangeable Mn(Ⅱ) by 51% and 73%, respectively. Organic anions oxalate, citrate, and malate reduced free Mn(Ⅱ) concentration in solution through formation of complexes with Mn(Ⅱ), efficiently decreasing exchangeable Mn(Ⅱ) on the roots; the decreases in exchangeable Mn(Ⅱ) on the roots were 30.9%, 19.7%, and 10.9%, respectively, which was consistent with the complexing ability of these organic anions with Mn(Ⅱ). Thus, exchangeable Mn(Ⅱ) was the dominant form of Mn(Ⅱ) on the roots and responsible for Mn(Ⅱ) toxicity to plants. The coexisting cations and organic anions reduced the exchangeable Mn(Ⅱ) content, and thus they could alleviate Mn(Ⅱ) toxicity to plants on acid soils.
Key Words:  acidic soils,cations,exchangeable Mn(II),organic anions,plant micronutrient,plant roots,toxicity alleviation
Citation: Liu Y, Li Z Y, Xu R K. 2019. Distribution of manganese(II) chemical forms on soybean roots and manganese(II) toxicity. Pedosphere. 29(5): 656-664.
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