Pedosphere 28(6): 905--912, 2018
ISSN 1002-0160/CN 32-1315/P
©2018 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Isolation, identification, and characterization of an aluminum-tolerant bacterium Burkholderia sp. SB1 from an acidic red soil
HUANG Shoucheng2, WANG Xiaodong3, LIU Xu1, HE Genhe1, WU Jichun4
1School of Life Sciences, Key Laboratory of Biology Diversity and Ecological Engineering of Jiangxi Province, Jinggangshan University, Ji'an 343009(China)
2School of Life Sciences, Anhui Science and Technology University, Fengyang 233100(China)
3Jiangxi Academy of Forestry, Nanchang 330013(China)
4Department of Hydrosciences, State Key Laboratory of Pollution Control and Resources Reuse, Nanjing University, Nanjing 210093(China)
ABSTRACT
      Aluminum (Al) toxicity usually occurs in acidic soils worldwide, which is detrimental to the growth of organisms. An Al-tolerant bacterium, SB1, was isolated from an acidic red soil of Chingkang Mountain, located in Jiangxi Province of China. Polyphasic analysis, including a 16S rDNA phylogenetic tree as well as morphological and physicochemical properties, revealed that the isolate was a gramnegative, rod-shaped bacterium, which was recognized as Burkholderia sp. SB1 and had extreme acidity tolerance (pH 2.2) and excellent Al resistance (270 mg L-1 Al3+). It could remove Al by up to 97.7% at a concentration of 54 mg L-1 Al3+. SB1 behavior under different temperatures and antibiotics was also examined. SB1 preferred moderate temperature conditions, ranging from 25 to 37℃, and exhibited notable resistance to multiple antibiotics (including ampicillin, streptomycin, and tetracycline), except for being sensitive to chloramphenicol. Therefore, as the first reported bacterium to possess favorable Al resistance and excellent Al removal, Burkholderia sp. SB1 can potentially be used as an agent for bioremediation of Al-contaminated acidic red soils.
Key Words:  antibiotics,bioremediation,Al resistance,Al toxicity,microbial community,plant growth-promoting bacteria,temperature
Citation: Huang S C, Wang X D, Liu X, He G H, Wu J C. 2018. Isolation, identification, and characterization of an aluminum-tolerant bacterium Burkholderia sp. SB1 from an acidic red soil. Pedosphere. 28(6):905-912.
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