Pedosphere 28(2): 241--254, 2018
ISSN 1002-0160/CN 32-1315/P
©2018 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Extracts from Marine Macroalgae and Opuntia ficus-indica Cladodes Enhance Halotolerance and Enzymatic Potential of Diazotrophic Rhizobacteria and Their Impact on Wheat Germination Under Salt Stress
Abdelwahab RAI1,2, Ameur CHERIF3, Cristina CRUZ4, Elhafid NABTI2
1Département de Microbiologie, Faculté des Sciences de la Nature et de la Vie, Université Ferhat Abbas, Sétif-1 19000 (Algérie)
2Université de Béjaïa, FSNV, Laboratoire de Maitrise des Energies Renouvelables (LMER), Equipe de Biomasse et Environnement, Targa Ouzemmour, Béjaïa 06000 (Algérie)
3LR Biotechnology and Bio-Geo Resources Valorization, Higher Institute for Biotechnology, University of Manouba, Sidi Thabet Biotechpole, 2020 Sidi Thabet, Ariana 2080 (Tunisia)
4cE3c-Centre for Ecology, Evolution and Environmental Changes, Campo Grande Bloco C-2, Piso 5, Sala 03, Lisboa 1749-016 (Portugal)
ABSTRACT
      Soil salinity, which affects more than 6% of the earth's land surface and more than 20% of its irrigated areas, is a major threat to agriculture. Diazotrophic bacteria are among the functional groups of soil microbiota that are threatened by this abiotic stress, as their activity is mostly inhibited by salt stress. Seventy bacterial strains with distinct characteristics were isolated from soils by using N-free Jensen's selective medium. Based on their ability to produce metabolites of agricultural interest, four strains were selected and identified as Flavobacterium johnsoniae, Pseudomonas putida, Achromobacter xylosoxidans, and Azotobacter chroococcum. The selected strains were grown at different NaCl concentrations (0-600 mmol L-1 in N-free broth and 0-2 000 mmol L-1 in Luria-Bertani medium) in the presence and absence of glycine betaine (GB), aqueous and hydro-alcoholic extracts from marine macroalgae, Ulva lactuca and Enteromorpha intestinalis, and Opuntia ficus-indica cladodes. The selected bacterial strains, GB, and the aforementioned extracts were tested for their ability to promote the germination of wheat (Triticum durum) seeds at 0-300 mmol L-1 NaCl. Compared with the results obtained with the synthetic osmoprotectant GB, the extracts from O. ficus-indica, U. lactuca, and E. intestinalis significantly promoted bacterial growth and seed germination under salt stress.
Key Words:  glycine betaine,Ulva lactuca,Enteromorpha intestinalis,plant growth-promoting rhizobacteria (PGPR),seed germination,Triticum durum
Citation: Rai A, Cherif A, Cruz C, Nabti E. 2018. Extracts from marine macroalgae and Opuntia ficus-indica cladodes enhance halotolerance and enzymatic potential of diazotrophic rhizobacteria and their impact on wheat germination under salt stress. Pedosphere. 28(2):241-254.
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