Pedosphere 27(6): 1073--1082, 2017
ISSN 1002-0160/CN 32-1315/P
©2017 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Autotoxicity of Phthalate Esters in Tobacco Root Exudates:Effects on Seed Germination and Seedling Growth
DENG Jiajun1,4, ZHANG Yanling2,5, HU Jiwei3, JIAO Jiaguo1, HU Feng1, LI Huixin1, ZHANG Shixiang2,5
1Soil Ecology Laboratory, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095 (China)
2Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001 (China)
3Guizhou Provincial Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang 550001 (China)
4Testing Center of Agro-Product Quality and Safety of Jiangxi Province, Nanchang 330046 (China)
5Key Laboratory of Eco-Environmental and Tobacco Leaf Quality of CNTC, Zhengzhou 450001 (China)
      Autotoxicity is one of the major factors that impede continuous cropping. It is defined as the toxic influence of chemicals released from one plant species on the germination and growth of individuals of the same species. Here, in order to exam the autotoxicity of tobacco root exudates, root exudates were collected from tobacco plants grown both in cultural solution and on natural soil. Using ultraperformance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry, main autotoxic chemical substances in the root exudates were identified. The autotoxic effects of suspected autotoxins on seed germination (including germination rate, germination potential, germination index, and vigor index) and seedling growth were analyzed. Dibutyl phthalate (or diisobutyl phthalate), dioctyl phthalate, and diisooctyl phthalate were identified in tobacco root exudates. It was observed that high concentrations (greater than 0.5 mmol L-1) of each identified phthalate ester caused significant (P < 0.05) inhibition of tobacco seed germination and seedling growth. It can be concluded that phthalate esters such as dibutyl phthalate, diisobutyl phthalate, and diisooctyl phthalate in tobacco root exudates may play an important role in tobacco autotoxicity.
Key Words:  autotoxins, germination index, germination potential, germination rate, vigor index
Citation: Deng J J, Zhang Y L, Hu J W, Jiao J G, Hu F, Li H X, Zhang S X. 2017. Autotoxicity of phthalate esters in tobacco root exudates: Effects on seed germination and seedling growth. Pedosphere. 27(6): 1073–1082.
View Full Text

Copyright © 2023 Editorial Committee of PEDOSPHERE. All rights reserved.
Address: P. O. Box 821, 71 East Beijing Road, Nanjing 210008, China    E-mail:
Technical support: Beijing E-Tiller Co.,Ltd.