Pedosphere 27(6): 1062--1072, 2017
ISSN 1002-0160/CN 32-1315/P
©2017 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Effects of Bacterial-Feeding Nematode Grazing and Tea Saponin Addition on the Enhanced Bioremediation of PyreneContaminated Soil Using Polycyclic Aromatic Hydrocarbon-Degrading Bacterial Strain
SUN Mingming1,2, LIU Kuan1, ZHAO Yuanchao1, TIAN Da1, YE Mao2, LIU Manqiang1, JIAO Jiaguo1, JIANG Xin2
1Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095 (China)
2Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)
Corresponding Author:SUN Mingming
      As one of the most widely distributed bacterial predators in the soil, the role of bacterivorous nematodes on the enhanced bioremediation of polycyclic aromatic hydrocarbon-contaminated soils is crucial, but remains to be investigated. A microcosm-level study was conducted to examine the effects of bacterial-feeding nematode grazing and tea saponin (TS) addition on bioremediation of a pyrene-contaminated soil enhanced by the polycyclic aromatic hydrocarbon (PAH)-degrading bacterial strain Sphingobium sp. PHE9. After 180 d of incubation, the highest pyrene dissipation (71.3%) was achieved through a combination of Sphingobium sp. PHE9 inoculation with nematode and TS addition. Meanwhile, high counts of culturable PAH-degrading bacteria, soil enzyme activity, and biodiversity indices were observed under the combined treatment, implying that the microbiological function of the contaminated soil was significantly restored. Additionally, the results of Tenax® extraction with the first-order three-compartment model indicated that rate-limiting factors varied among treatments. The lack of degrading microorganisms was the main rate-limiting factor for the treatments involving TS/nematode addition, and inadequate bioaccessible pyrene was the vital rate-limiting factor in the treatments involving Sphingobium sp. PHE9 inoculation. The proposed combined clean-up strategy proved to be a promising bioremediation technology for aged pyrene-contaminated soils.
Key Words:  desorbing fraction, desorption rate constant, physiological profile of soil microbial community, Shannon-Weaver index, Simpson index, soil microbial activity, Sphingobium sp. PHE9
Citation: Sun M M, Liu K, Zhao Y C, Tian D, Ye M, Liu M Q, Jiao J G, Jiang X. 2017. Effects of bacterial-feeding nematode grazing and tea saponin addition on the enhanced bioremediation of pyrene-contaminated soil using polycyclic aromatic hydrocarbon-degrading bacterial strain. Pedosphere. 27(6): 1062–1072.
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