Pedosphere 27(5): 968--973, 2017
ISSN 1002-0160/CN 32-1315/P
©2017 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Evaluating soil dissolved organic matter extraction using three-dimensional excitation-emission matrix fluorescence spectroscopy
XIE Wenming1, ZHANG Shanshan2, RUAN Lin2, YANG Mingyue2, SHI Weiming1, ZHANG Hailin3, LI Weihua4
1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)
2College of Environment, Hohai University, Nanjing 210098 (China)
3Department of Plant and Soil Sciences, Oklahoma State University, Stillwater OK 74078-6028 (USA)
4School of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601 (China)
ABSTRACT
      Soil dissolved organic matter (DOM) consists of many organic compounds and plays an important role in contaminant transport in the ecosystem. However, the effects of different extraction conditions on contents of different DOM components are poorly understood. We extracted DOM from three soils using different extraction times, solid to liquid ratios (SLR), and extraction solution (KCl) concentrations, and evaluated the extractions using three-dimensional excitation-emission matrix (EEM) fluorescence spectroscopy. The sum of the contents of the main DOM components (protein-, fulvic-, and humic-like compounds) increased by 0.6-3.5, 2.5-3.9, and 0.11-0.37 times (P < 0.05) when SLR decreased from 1:2 to 1:10, the extraction solution concentration increased from 0.01 to 1.5 mol L-1, and extraction time increased from 10 to 300 min, respectively. The three-dimensional EEM fluorescence spectroscopy is a useful tool to characterize the components of DOM and evaluate DOM extraction in soils.
Key Words:  dissolved organic matter components, extraction conditions, extraction time, KCl concentration, solid to liquid ratio
Citation: Xie, W., Zhang, S., Ruan, L., Yang, M., Shi, W., Zhang, H. and Li, W. 2017. Evaluating soil dissolved organic matter extraction using three-dimensional excitation-emission matrix fluorescence spectroscopy. Pedosphere. 27(5): 968-973.
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