Pedosphere 27(5): 832--845, 2017
ISSN 1002-0160/CN 32-1315/P
©2017 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Amazonian Dark Earth and its black carbon particles harbor different fungal abundance and diversity
Adriano Reis LUCHETA1, Fabiana de SOUZA CANNAVAN2, Siu Mui TSAI2, Eiko Eurya KURAMAE1
1Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO/KNAW), Wageningen 6708PB (The Netherlands)
2Centro de Energia Nuclear na Agricultura (CENA), University of São Paulo (USP), Piracicaba, São Paulo 13400-970 (Brazil)
ABSTRACT
      Amazonian Dark Earth (ADE) is a highly fertile soil of anthropogenic origin characterized by high levels of charred black carbon (BC). It is considered a model of fertility; however, knowledge on the fungal community structure and diversity inhabiting ADE and its BC particles is scarce. Fungal community structure and diversity of ADE and its BC from four sites under different land uses (three agricultural systems and a secondary pristine forest) in the Brazilian Central Amazon were evaluated by 18S rRNA gene pyrosequencing. Fungal communities in ADE and BC were dissimilar and showed differential abundances of fungal operational taxonomic units (OTUs). Estimated fungal species richness (abundance-based coverage estimate and Chao-1 index) and diversity estimators (Shannon and Simpson's reciprocal indices) were higher in ADE than in BC in all agricultural areas. No differences were observed in those parameters in ADE and BC samples from the secondary forest. Pezizomycotina fungi and OTUs assigned to Cordyceps confragosa, Acremonium vitellinum, Camarops microspora, and Hirsutella rhossiliensis were more abundant in BC particles than in ADE. These findings represent a breakthrough in our understanding of fungal communities in BC particles from ADE, and will be valuable in future studies considering biochar application in soil.
Key Words:  biochar, fungal community, high-throughput DNA sequencing, 18S rRNA gene, terra preta
Citation: Reis, L., Souza, C., Mui, T. and Eurya, K. 2017. Amazonian Dark Earth and its black carbon particles harbor different fungal abundance and diversity. Pedosphere. 27(5): 832-845.
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