Pedosphere 27(1): 165--171, 2017
ISSN 1002-0160/CN 32-1315/P
©2017 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Effects of bacterial-feeding nematodes and glucose on phenanthrene removal by Pseudomonas putida
JING Yong-Ping1,2,3, LI Yan1,2, LIU Zhao-Hui4, ZHANG Ying-Peng1,2, LIU Ping1, SUN Ming1 and J. LUO5
1Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Sciences, Jinan 250100 (China)
2Key Laboratory of Agro-Environment in Huang-Huai-Hai Plain, Ministry of Agriculture, Jinan 250100 (China)
3Shandong Provincial Key Laboratory of Plant Nutrition and Fertilizer, Jinan 250100 (China)
4Shandong Academy of Agricultural Sciences, Jinan 250100 (China)
5AgResearch, Hamilton 3240 (New Zealand)
ABSTRACT
      Bacterial-feeding nematodes can promote the bacterial activity through feeding. Bacterial abundance and their activity affect the degradation of polycyclic aromatic hydrocarbons (PAH) such as phenanthrene. The effects of bacterial-feeding nematodes, bacteria, and their interactions on the degradation of phenanthrene with or without glucose were studied through a microcosm experiment. The results showed that up to 57.0% of phenanthrene in mineral medium contaminated with phenanthrene was degraded in the control with bacteria alone and feeding bacteria with the presence of nematodes and/or glucose increased the degradation of phenanthrene by 25.6% to 36.6%. Although both nematode and bacteria abundance decreased gradually, catechol 2,3-dioxygenase (C23O) activity increased during the incubation period. Compared with bacteria alone, the presence of nematodes significantly increased C23O activity as well as the abundance of bacteria; this effect was more pronounced when glucose was present. The results imply that nematodes might promote the removal of phenanthrene from medium by stimulating bacteria and C23O activities.
Key Words:  biodegradation, biostimulation, catechol 2,3-dioxygenase, enzyme activity, polycyclic aromatic hydrocarbons
Citation: Jing, Y. P., Li, Y., Liu, Z. H., Zhang, Y. P., Liu, P., Sun, M. and Luo, J. 2017. Effects of bacterial-feeding nematodes and glucose on phenanthrene removal by Pseudomonas putida. Pedosphere. 27(1): 165-171.
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