Pedosphere 26(3): 273--281, 2016
ISSN 1002-0160/CN 32-1315/P
©2016 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Integration of chemical methods and biomarkers for assessment of chlorimuron-ethyl bioavailability in soil
REN Wen-Jie1,2, WANG Mei-E3, ZHOU Qi-Xing4 and TENG Ying1
1Key Laboratory of Soil Environment and Pollution Remediation of Chinese Academy of Sciences, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)
2Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016 (China)
3State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085 (China)
4Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), College of Environmental Science and Engineering, Nankai University, Tianjin 300071 (China)
      Bioavailability is a critical factor for assessing the environmental risk of organic pollutants in soil. In this study, extractions with 3 different solvents, including 2 aqueous solutions, calcium chloride (CaCl2) and a phosphate buffer solution (PBS), and a mixture of aqueous solution and organic solvent, a PBS-methanol (8:2, volume/volume) mixture (PBS-M), were performed to assess the bioavailability of chlorimuron-ethyl in soil in comparison to a battery of toxicity tests in wheat seedlings. The results indicated that the peroxidase (POD) activity in wheat leaves after 7 d of exposure was one of the sensitive biomarkers of chlorimuron-ethyl in soil. The extractability of chlorimuron-ethyl by all the 3 solvents decreased with exposure time, and the rate of decrease of the PBS-M extraction between 1 and 7 d of exposure was substantially higher than those of the aqueous solution extractions. Chlorimuron-ethyl gradually changed from a water-soluble form into a soil organic matter (SOM)-bound form in the soil. The PBS extraction correlated best with the POD activity in the leaves after 7 d of exposure.
Key Words:  chemical extraction, environmental risk, organic pollutants, organic solvent, peroxidase activity, phosphate buffer solution, toxicity test
Citation: Ren, W. J., WANG Mei-E, Zhou, Q. X. and Teng, Y. 2016. Integration of chemical methods and biomarkers for assessment of chlorimuron-ethyl bioavailability in soil. Pedosphere. 26(3): 273-281.
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