Pedosphere 24(6): 799--807, 2014
ISSN 1002-0160/CN 32-1315/P
©2014 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Isolation and characterization of chlorothalonil-degrading bacterial strain H4 and its potential for remediation of contaminated soil |
ZHANG Man-Yun1,2, TENG Ying1 , ZHU Ye1,2, WANG Jun1, LUO Yong-Ming1, P. CHRISTIE1, LI Zhen-Gao1 and T. K. UDEIGWE3 |
1Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)
2University of the Chinese Academy of Sciences, Beijing 100049 (China)
3
Department of Plant and Soil Science, Texas Tech. University, Lubbock, TX 79409 (USA) |
| ABSTRACT |
| A chlorothalonil (CTN)-degrading bacterial strain H4 was isolated in this study from a contaminated soil by continuous enrichment culture to identify its characteristics and to investigate its potential for remediation of CTN in contaminated soil. Based on the morphological, physiological and biochemical tests and 16S rDNA sequence analysis, the strain was identified as Stenotrophomonas sp. After liquid culture for 7 d, 82.2% of CTN was removed by strain H4. The isolate could degrade CTN over a broad range of temperatures and pH values, and the optimum conditions for H4 degradation were pH 7.0 and 30 °C. Reintroduction of the bacteria into artificially contaminated soil resulted in substantial removal of CTN (> 50%) after incubation for 14 d. Soil samples treated by H4 showed significant increases (P < 0.05) in soil dehydrogenase activity, soil polyphenol oxidase activity, average well-color development obtained by the Biolog Eco plateTM assay and Shannon-Weaver index, compared with the control. Strain H4 might be a promising candidate for application in the bioremediation of CTN-contaminated soils. |
| Key Words: biodegradation, fungicide, soil bioremediation, Stenotrophomonas sp. |
| Citation: Zhang, M. Y., Teng, Y., Zhu, Y., Wang, J., Luo, Y. M., Christie, P., Li, Z. G. and Udeigwe, T. K. 2014. Isolation and characterization of chlorothalonil-degrading bacterial strain H4 and its potential for remediation of contaminated soil. Pedosphere. 24(6): 799-807. |
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