Pedosphere 22(6): 735--745, 2012
ISSN 1002-0160/CN 32-1315/P
©2012 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Temporal and tissue-specific expression of tomato 14-3-3 gene family in response to phosphorus deficiency
XU Wei-Feng1, SHI Wei-Ming1 and YAN Feng2
1 State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China)
2 Institute of Crop Science and Plant Breeding, Justus-Liebig University, Ludwigstrasse 23, 35390 Giessen (Germany)
ABSTRACT
      Plants adapt to phosphorus (P) deficiency through a complex of biological processes and many genes are involved.Tomato ( Solanum lycopersicum L. ‘Hezuo906') plants were selected to grown hydroponically to study the temporal and spatial gene expression patterns of the 14-3-3 gene family and their roles in response to P deficiency in tomato plants. Using real-time reverse-transcriptase polymerase chain reaction (RT-PCR), we investigated the expression profiles in different tissues (root, stem and leaf) at short-term and long-term P-deficient stress phases. Results revealed that i) four members of 14-3-3 gene family (TFT1, TFT4, TFT6 and TFT7) were involved in the adaptation of tomato plants to P deficiency, ii) TFT7 responded quickly to P deficiency in the root, while TFT6 responded slowly to P deficiency in the leaf, iii) expression response of TFT4 to P-deficient stress was widely distributed in different tissues (root, stem and leaf) while TFT8 only displayed stem-specific expression, and iv) temporal and tissues-specific expression patterns to P deficiency suggested that isoform specificity existed in tomato 14-3-3 gene family. We propose that TFT7 (one member of ε-like group in tomato 14-3-3 family) is the early responsive gene and may play a role in the adaptation of tomato plants to short-term P deficiency, while TFT6 (one member of non-ε group in tomato 14-3-3 family) is the later responsive gene and may play a role in the adaptation of tomato plants to long-term P deficiency.
Key Words:  isoform specificity, mRNA, P starvation, primer pairs, real-time RT-PCR
Citation: Xu, W. F., Shi, W. M. and Yan, F. 2012. Temporal and tissue-specific expression of tomato 14-3-3 gene family in response to phosphorus deficiency. Pedosphere. 22(6): 735-745.
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