Pedosphere 22(5): 673--680, 2012
ISSN 1002-0160/CN 32-1315/P
©2012 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Diversity and Characterization of Potential H2-Dependent Fe(Ⅲ)-Reducing Bacteria in Paddy Soils |
| LI Hui-Juan1,2, PENG Jing-Jing1,2, LI Hong-Bo1,2 |
| 1.Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
| ABSTRACT |
| Microbial ferric iron reduction,with organic carbon or hydrogen as the effectron donor,is one of the most important biogeochemica processes in anoxic paddy soils;however,the diversity and community structure of hydrogen-dependent dissimilatory iron-reducers remain unknown.Potential H2-dependent Fe(Ⅲ)-reducing bacteria in paddy soils were explored using enrichment cultures with ferrihydrite or goethite as the effectron acceptor and hydrogen as the effectron donor.Terminal restriction fragment length polymorphism (T-RFLP)analysis and cloning/sequencing were conducted to reveal bacterial community structure.Results showed that Geobacter and Clostridium were the dominant bacteria in the enrichment cultures.Fe(Ⅲ)oxide mineral phases showed a strong effect on the community structure;Geobacter and Clostridium were dominant in the ferrihydrite treatment,while Clostridium spp.were dominant in the goethite treatment.These suggested that H2-dependent Fe(Ⅲ)-reducing bacteria might be widely distributed in paddy soils and that besides Geobacter,Clostridium spp.might also be an important group of H2-dependent Fe(Ⅲ)-reducing microorganisms. Key Words:bacterial community structure,Clostridium,enrichment culture,Geobacter,terminal restriction fragment length poly morphism |
| Key Words: bacterial community structure,Clostridium,enrichment culture,Geobacter,terminal restriction fragment length polymorphism |
| Citation: Li, H. J., Peng, J. J. and Li, H. B. 2012. Diversity and Characterization of Potential H2-Dependent Fe(Ⅲ)-Reducing Bacteria in Paddy Soils. Pedosphere. 22(5): 673-680. |
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