Pedosphere 22(2): 201--205, 2012
ISSN 1002-0160/CN 32-1315/P
©2012 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| An attempt to quantify Cu-resistant microorganisms in a paddy soil from Jiaxing, China |
LI Xiao-Fang1,2, YIN Hong-Bin3 and SU Jian-Qiang1 |
1 Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361003 (China)
2 Centre for Mined Land Rehabilitation, Sustainable Minerals Institute, the University of Queensland, St Lucia Qld 4072 (Australia)
3 Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085 (China) |
| ABSTRACT |
| Understanding the mechanisms of Cu pollution-induced community tolerance (PICT) in soil requires the characterization of Cu-resistant microorganisms at a community level using modern molecular tools. A primer pair (copAF2010 (5'-TGCAC CTGAC VGGSC AYAT-3')copAR2333 (5'-GVACT TCRCG GAACA TRCC-3')) tentatively targeting Pseudomonas-like Cu-resistant microorganisms was designed in this study. The specification of the primers was tested through conventional polymerase chain reaction (PCR) and the construction of a Pseudomonas-like copA gene fragment library, and then the primers were used to quantify the Cu-resistant microorganisms using quantitative PCR technique. A significant increase of Cu-resistant microorganisms targeted by the primers was observed in a paddy soil from Jiaxing, China which has been exposed to one-year Cu contamination. The results provided direct evidence for Cu PICT in the soil, and the quantification method developed in this study has the potential to be used as a molecular assay for soil Cu pollution. |
| Key Words: copA, Cu contamination, pollution-induced community tolerance (PICT), quantitative PCR |
| Citation: Li, X. F., Yin, H. B. and Su, J. Q. 2012. An attempt to quantify Cu-resistant microorganisms in a paddy soil from Jiaxing, China. Pedosphere. 22(2): 201-205. |
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