Pedosphere 21(1): 31--36, 2011
ISSN 1002-0160/CN 32-1315/P
©2011 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Biodegradation of pentachloronitroben-zene by Labrys portucalensis pcnb-21 isolated from polluted soil |
LI Rong, ZHENG Jin-Wei, NI Bin, CHEN Kai, YANG Xiu-Juan, LI Shun-Peng and JIANG Jian-Dong |
| Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095 (China) |
| ABSTRACT |
| A bacterial strain, pcnb-21, capable of degrading pentachloronitrobenzene(PCNB) under aerobic and anoxic conditions, was isolated from a long-term PCNB-polluted soil by an enrichment culture technique and identified as Labrys portucalensis based upon its morphological, physiological and biochemical properties, as well as 16S rRNA gene sequence analysis. Effects of different factors, such as temperature and pH, on PCNB biodegradation were studied. Strain pcnb-21 efficiently degraded PCNB at temperatures from 20 to 30 ℃ and initial pH values from 4 to 7, which might be the first time that a Labrys strain was found capable of efficiently degrading PCNB. The degradation of PCNB was affected by oxygen, and the degradation decreased with increasing aeration. Exogenous electron donors such as glucose, lactic acid and succinic acid promoted the biodegradation of PCNB, while electron acceptors such as sodium nitrite, sodium sulfate, sodium nitrate and sodium sulfate inhibited PCNB biodegradation. The degradation of PCNB in sterile and non-sterile soils by a green fluorescent protein(GFP)-labeled strain, pcnb-21-gfp, was also studied. Cells of pcnb-21-gfp efficiently degraded 100 mg kg-1 PCNB in sterile and non-sterile soils and could not be detected after 42 days. Strain pcnb-21 might be useful in bioremediating PCNB-polluted soils and environment. |
| Key Words: aeration, bacterial strain, electron acceptors, electron donors, sterile soil |
| Citation: Li, R., Zheng, J. W., Ni, B., Chen, K., Yang, X. J., Li, S. P. and Jiang, J. D. 2011. Biodegradation of pentachloronitroben-zene by Labrys portucalensis pcnb-21 isolated from polluted soil. Pedosphere. 21(1): 31-36. |
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