Pedosphere 20(1): 111--119, 2010
ISSN 1002-0160/CN 32-1315/P
©2010 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Isolation and characterization of a bensulfuron-methyl-degrading strain L1 of Bacillus
LIN Xiao-Yan1,2, WANG Yi1, WANG Hai-Long3, T. CHIRKO4, DING Hai-Tao1 and ZHAO Yu-Hua1
1 Department of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058 (China)
2 China National Rice Research Institute, Hangzhou 310006 (China)
3 Ensis, Rotorua Private Bag 3020 (New Zealand)
4 College of Environmental Sciences and Technology, Zhejiang Forestry University, Lin'an 311300 (China)
      The objectives of this study were to isolate a bensulfuron-methyl (BSM)-degrading strain of Bacillus spp. and to eval-uate its effectiveness in remediation of a BSM-contaminated soil. A BSM-degrading bacterium, strain L1, was successfully isolated in this study. Strain L1 was identified as Bacillus megaterium based on its morphological, physiological, and biochemical properties, G+C content, phylogenetic similarity of 16S rDNA, and fatty acid composition. Two experiments were used to examine BSM degradation by strain L1. When BSM was used as a sole carbon source in a mineral salt medium, the average degradation rate of BSM by strain L1 was 12.8%, which suggested that the strain was able to utilize BSM as a sole carbon and energy source. Supplement of yeast extract (200 mg L-1) significantly (P ≤ 0.01) accelerated the degradation of BSM by strain L1. Almost complete degradation (97.7%) of BSM could be achieved in 84 h with addition of yeast extract. In addition, in a sterile soil with 50 mg L-1 BSM, BSM degradation rate by strain L1 was 94.3% in 42 d, indicating the potential of using microbes for the remediation of BSM-contaminated soils in fields.
Key Words:  biodegradation, carbon source, phylogenetic analysis, 16S rDNA
Citation: Lin, X. Y., Wang, Y., Wang, H. L., Chirko, T., Ding, H. T. and Zhao, Y. H. 2010. Isolation and characterization of a bensulfuron-methyl-degrading strain L1 of Bacillus. Pedosphere. 20(1): 111-119.
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