Pedosphere 19(2): 238--243, 2009
ISSN 1002-0160/CN 32-1315/P
©2009 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Isolation and characterization of a methomyl-degrading Paracoccus sp. mdw-1 |
XU Jing-Liang 1,2, WU Jun1, WANG Zhi-Chun1, WANG Kun1, LI Meng-Ying1, JIANG Jian-Dong1, HE Jian1 and LI Shun-Peng1 |
1 Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095 (China). E-mail:xjl@ms.giec.ac.cn
2 Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy and Gas Hydrate, Chinese Academy of Sciences, Guangzhou 510640 (China) |
| ABSTRACT |
| Methomyl, an extremely toxic pesticide, is widely used in agriculture. A strain named mdw-1 capable of degrading methomyl rapidly was successfully isolated from activated sludge in this study. It could utilize methomyl as the sole carbon or nitrogen source. The optimal temperature and medium pH for its growth and methomyl biodegradation were 30-C and 7.0, respectively. It was identified as a Paracoccus sp. according to its morphological features, physiological and biochemical characteristics, and phylogenetic analysis based on the sequence of 16S rDNA. Gas chromatography-mass spectrometry (GC-MS) analysis showed that methomyl could be completely transformed to S-methyl-N-hydroxythioacetamidate in 10 h of incubation with the isolate mdw-1. |
| Key Words: methomyl degradation, Paracoccus sp., S-methyl-N-hydroxythioacetamidate, 16S rDNA, strain mdw-1 |
| Citation: Xu, J. L., Wu, J., Wang, Z. C., Wang, K., Li, M. Y., Jiang, J. D., He, J. and Li, S. P. 2009. Isolation and characterization of a methomyl-degrading Paracoccus sp. mdw-1. Pedosphere. 19(2): 238-243. |
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