Pedosphere 19(2): 156--165, 2009
ISSN 1002-0160/CN 32-1315/P
©2009 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Bacterial community structure in a mollisol under long-term natural restoration, cropping, and bare fallow history estimated by PCR-DGGE |
WANG Guang-Hua , JIN Jian, LIU Jun-Jie, CHEN Xue-Li, LIU Ju-Dong and LIU Xiao-Bing |
| Key Laboratory of Black Soil Ecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081 (China). E-mail:guanghuawang@hotmail.com |
| ABSTRACT |
| Soil microbial biomass and community structures are commonly used as indicators for soil quality and fertility. A investigation was performed to study the effects of long-term natural restoration, cropping, and bare fallow managements on the soil microbial biomass and bacterial community structures in depths of 0-10, 20-30, and 40-50 cm in a black soil (Mollisol). Microbial biomass was estimated from chloroform fumigation-extraction, and bacterial community structures were determined by analysis of 16S rDNA using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Experimental results showed that microbial biomass significantly declined with soil depth in the managements of restoration and cropping, but not in the bare fallow. DGGE profiles indicated that the band number in top 0-10 cm soils was less than that in depth of 20-30 or 40-50 cm. These suggested that the microbial population was high but the bacterial community structure was simple in the topsoil. Cluster and principle component analysis based on DGGE banding patterns showed that the bacterial community structure was affected by soil depth more primarily than by managements, and the succession of bacterial community as increase of soil depth has a similar tendency in the three managements. Fourteen predominating DGGE bands were excised and sequenced, in which 6 bands were identified as the taxa of Verrucomicrobia, 2 bands as Actinobacteria, 2 bands as α-Proteobacteria, and the other 4 bands as δ-Proteobacteria, Acidobacteria, Nitrospira, and unclassified bacteria. In addition, the sequences of 11 DGGE bands were closely related to uncultured bacteria. Thus, the bacterial community structure in black soil was stable, and the predominating bacterial groups were uncultured. |
| Key Words: bacterial community, black soil, denaturing gradient gel electrophoresis (DGGE), polymerase chain reaction (PCR), soil depth |
| Citation: Wang, G. H., Jin, J., Liu, J. J., Chen, X. L., Liu, J. D. and Liu, X. B. 2009. Bacterial community structure in a mollisol under long-term natural restoration, cropping, and bare fallow history estimated by PCR-DGGE. Pedosphere. 19(2): 156-165. |
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