Pedosphere 18(5): 621--627, 2008
ISSN 1002-0160/CN 32-1315/P
©2008 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| PCR-DGGE analysis of nematode diversity in Cu-contaminated soil |
WANG Shi-Bin, LI Qi , LIANG Wen-Ju, JIANG Yong and JIANG Si-Wei |
| Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016 (China). E-mail: liq@iae.ac.cn |
| ABSTRACT |
| A wheat pot experiment was conducted under greenhouse conditions to assess the effect of copper contamination on soil nematode diversity by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method and morphological analysis. The soil was treated with CuSO4·5H2O at the following concentrations:0, 50, 100, 200, 400, and 800 mg kg-1 dry soil, and the soil samples were collected at wheat jointing and ripening stages. Nematode diversity index (H') from morphological analysis showed no difference between the control and the treated samples in either of the sampling dates. At the wheat ripening stage, nematode diversity obtained by the PCR-DGGE method decreased noticeably in the Cu800 treatment in comparison with the control. With optimization of the method of nematode DNA extraction, PCR-DGGE could give more information on nematode genera, and the intensity of the bands could reffect the abundance of nematode genera in the assemblage. The PCR-DGGE method proved promising in distinguishing nematode diversity in heavy metal contaminated soil. |
| Key Words: copper contamination, morphological analysis, nematode diversity, PCR-DGGE |
| Citation: Wang, S. B., Li, Q., Liang, W. J., Jiang, Y. and Jiang, S. W. 2008. PCR-DGGE analysis of nematode diversity in Cu-contaminated soil. Pedosphere. 18(5): 621-627. |
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