Pedosphere 18(1): 102--112, 2008
ISSN 1002-0160/CN 32-1315/P
©2008 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Strain mesorhizobium sp. CCNWGX035: A stress-tolerant isolate from Glycyrrhiza glabra displaying a wide host range of nodulation
WEI Ge-Hong1, YANG Xue-Ying1, ZHANG Zhi-Xin1, YANG Ya-Zhen1 and K. LINDSRÖM2
1 Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Life Science, Northwest A&F University, Yangling Shaanxi 715100 (China). E-mail: weigehong@yahoo.com.cn
2 Department of Applied Chemistry and Microbiology, Biocenter 1, Fin-0014 University of Helsinki (Finland)
ABSTRACT
      Thirty-nine rhizobial isolates were isolated from the root nodules of Glycyrrhiza uralensis and Glycyrrhiza glabra, growing in the arid and semiarid regions of northwestern China, to test their taxonomic position and stress tolerance and to select one promising putative inoculant strain for further studies. On the basis of 113 physiological and biochemical characteristics, the isolates were clustered into three groups. One isolate CCNWGX035 was found to have high tolerance to NaCl, pH, and temperature. By sequencing the 16S rDNA, isolate CCNWGX035 was placed in genus Mesorhizobium. Nodulation tests demonstrated that the isolate not only formed nitrogen-fixing nodules on its original host plant Glycyrrhiza glabra, but also on Sophora viciifolia, Lotus corniculatus, Trifolium repens, Melilotus suaveolens, and Sophora alopecuroides. On the basis of sequence analysis of the nodA gene, isolate CCNWGX035 was closely related to strains of the genus Mesorhizobium, exhibiting some novel characteristics of root nodule bacteria.
Key Words:  Glycyrrhiza, host range, Mesorhizobium, nodA, stress tolerance
Citation: Wei, G. H., Yang, X. Y., Zhang, Z. X., Yang, Y. Z. and LindsrÖm, K. 2008. Strain mesorhizobium sp. CCNWGX035: A stress-tolerant isolate from Glycyrrhiza glabra displaying a wide host range of nodulation. Pedosphere. 18(1): 102-112.
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