Pedosphere 17(5): 624--629, 2007
ISSN 1002-0160/CN 32-1315/P
©2007 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Enhanced biological phosphorus removal with Pseudomonas putida GM6 from activated sludge
CAI Tian-Ming1,2, GUAN Li-Bo1,2, CHEN Li-Wei1,2, CAI Shu1,2, LI Xiao-Dan1,2, CUI Zhong-Li1,2 and LI Shun-Peng1,2
1 Department of Microbiology, College of Life Science, Nanjing Agricultural University, Nanjing 210095 (China). E-mail:
2 Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing 210095 (China)
      The enhanced biological phosphorus removal (EBPR) method is widely adopted for phosphorus removal from waste-water, yet little is known about its microbiological and molecular mechanisms. Therefore, it is difficult to predict and control the deterioration of the EBPR process in a large-scale municipal sewage treatment plant. This study used a novel strain isolated in the laboratory, Pseudomonas putida GM6, which had a high phosphate accumulating ability and could recover rapidly from the deteriorated system and enhance the capability of phosphorus removal in activated sludge. Strain GM6 marked with gfp gene, which was called GMTR, was delivered into a bench-scale sequencing batch reactor (SBR) of low efficiency, to investigate the colonization of GMTR and removal of phosphorus. After 21 days, the proportion of GMTR in the total bacteria of the shdge reached 9.2%, whereas the phosphorus removal rate was 96%, with an effluent concentration of about 0.2 mg L-1. In the reactor with the addition of GMTR, phosphorus was removed quickly, in 1h under anaerobic conditions, and in 2h under aerobic conditions. These evidences were characteristic of EBPR processes, Field testing was conducted at a hospital sewage treatment facility with low phosphorus removal capability. Twenty-one days after Pseudornonas putida GM6 was added, effluent phosphorus concentration remained around 0.3 mg L-1, corresponding to a removal rate of 96.8%. It was therefore demonstrated that Pseudornonas putida GM6 could be used for a quick startup and enhancement of wastewater biological phosphorus removal, which provided a scientific basis for potential large-scale engineering application.
Key Words:  activated sludge, colonization, enhanced biological phosphorus removal, Pseudomonas putida GM6
Citation: Cai, T. M., Guan, L. B., Chen, L. W., Cai, S., Li, X. D., Cui, Z. L. and Li, S. P. 2007. Enhanced biological phosphorus removal with Pseudomonas putida GM6 from activated sludge. Pedosphere. 17(5): 624-629.
View Full Text

Copyright © 2023 Editorial Committee of PEDOSPHERE. All rights reserved.
Address: P. O. Box 821, 71 East Beijing Road, Nanjing 210008, China    E-mail:
Technical support: Beijing E-Tiller Co.,Ltd.