Pedosphere 16(1): 64--71, 2006
ISSN 1002-0160/CN 32-1315/P
©2006 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Isolation and characterization of a dichlorvos-degrading strain DDV-1 of Ochrobactrum sp. |
ZHANG Xiao-Hua1,2 , ZHANG Guo-Shun1, ZHANG Zhong-Hui1, XU Jian-Hong1 and LI Shun-Peng1 |
1 Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095 (China). E-mail: xhzh624@sohu.com
2 Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 212400 (China) |
| ABSTRACT |
| The objective of this research was to isolate a dichlorvos (2,2-dichlorovinyl dimethyl phosphate)-degrading strain of Ochrobactrum sp., and determine its effectiveness in remediation of a dichlorvos-contaminated soil. A dichlorvos-degrading bacterium (strain DDV-1) was successfully isolated and identified as an Ochrobactrum sp. based on its 16S rDNA sequence analysis. Strain DDV-1 was able to utilize dichlorvos as a sole carbon source, and the optimal pH and temperature for its cell growth and degradation were 7.0 and 30 ℃, respectively. Also, the growth and degradation of strain DDV-1 showed the same response to dissolved oxygen. In addition, the soil degradation test indicated that in soil spiked with 100 mg L-1 or 500 mg L-1 dichlorvos and inoculated with 0.5% or 1.0% (v/v) strain DDV-1, complete degradation of dichlorvos could be achieved in 24 h. The present study showed that strain DDV-1 was a fast dichlorvos-degrading bacterium in soil. However, further research will be needed to clarify the degradation pathway and the properties of the key enzymes involved in its biodegradation. |
| Key Words: degradation, dichlorvos, 16S rDNA, soil test, strain DDV-1 |
| Citation: Zhang, X. H., Zhang, G. S., Zhang, Z. H., Xu, J. H. and Li, S. P. 2006. Isolation and characterization of a dichlorvos-degrading strain DDV-1 of Ochrobactrum sp.. Pedosphere. 16(1): 64-71. |
| |
|
View Full Text
|
|
|
|
