Pedosphere 9(2): 97--104, 1999
ISSN 1002-0160/CN 32-1315/P
©1999 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Determination of organic acids in root exudates by high performance liquid chromatography: Ⅲ. Effects of interfering factors |
| SHEN Jian-Bo, ZHANG Fu-Suo and MAO Da-Ru |
| Department of Plant Nutrition, China Agricultural University, Beijing 100094 (China) |
| ABSTRACT |
| A solution culture experiment was conducted to investigate the effects of collection time and interfering ions on separation and determination of low-molecular-weight organic acids in root exudates of soyabean using the method for directly collecting root exudates. The suitable collection time of root exudates and the interfering ions affecting organic acid determination were determined. The method for removing the interfering ions was established and analysed. The release amount of root exudates increased with the increase of collection time from 0 to 120 min but decreased with increasing of collection time from 120 to 240 min. The maximum exuding amounts of organic acids were observed in root exudates at the collection time of 120 min. There was a significant difference of organic acid components between the treatments of collection time of 120 and 240 min. Citric acid was found only in the treatment of 120 min collection time. NO3- was the main interfering ion in organic acid determination and had the same retention time as oxalic acid. Anion exchange resin (SAX) properly treated by HPLC solvent could remove NO3- anion in sample solution of root exudates, thus enhancing the recoveries of organic acids in root exudates. There was no significant effect of the chemicals added into sample solution such as H3PO4, SAX and KNO3 on the retention time of organic acids. |
| Key Words: collection time, interfering factor, low-molecular-weight organic acid, root exudates |
| Citation: Shen, J. B., Zhang, F. S. and Mao, D. R. 1999. Determination of organic acids in root exudates by high performance liquid chromatography: Ⅲ. Effects of interfering factors. Pedosphere. 9(2): 97-104. |
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