Pedosphere 7(4): 303--310, 1997
ISSN 1002-0160/CN 32-1315/P
©1997 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Effect of replacement of headspace gas on N2O and CO2 emissions in anaerobic incubation of soil |
| CHEN Tong-Bin1, S. STRUWE2 and A. KJΦLLERZ2 |
1 Station for Ampecolopy and Environmental Technolopy, Institute of Geogmphy, Chinese Academy of Sciences, Beijing 100101 (China)
2 Department of General Microbiology, University of Copenhagen, SΦlvgade 83H, DK-1307 Copenhagen (Denmark) |
| ABSTRACT |
| To study effect of C2H2 and change of headspace gas on N2O emission, denitrification, as well as CO2 emission, slurries of an agricultural soil were anaerobically incubated for 7 days at 25 ℃. Both N2O reduction and CO2 emissions were inhibited by the addition of 100 mL L-1 of C2H2. However, the inhibition to CO2 emission was alleviated by the replacement of headspace gas, and the N2O emission was enhanced by the replacement. Acetylene disappeared evidently from the soil slurries during the incubation. Consequently results obtained from the traditional C2H2 blocking technique for determination of denitrification rate, especially in a long-time incubation, should be explained with care because of its side effect existing in the incubation environments without change of headspace gas. To reduce the possible side effect on the processes other than denitrification, it is suggested that headspace gas should be replaced several times during a long-time incubation. |
| Key Words: acetylene, anaerobic incubation, CO2 emission, headspace gas, N2O emission |
| Citation: Chen, T. B., Struwe, S. and Kjφllerz, A. 1997. Effect of replacement of headspace gas on N2O and CO2 emissions in anaerobic incubation of soil. Pedosphere. 7(4): 303-310. |
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