Pedosphere 6(3): 209--216, 1996
ISSN 1002-0160/CN 32-1315/P
©1996 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Fractionation of soil selenium with relation to Se availability to plants
HE Zhen-Li1, YANG Xiao-E1, ZHU Zu-Xiang1, XIA Wei-Ping2, PAN Jian-Ming2 and LIU Xiao-Ya2
1 Department of Land Use and Applied Chemistry, Zhejiang Agricultural University, Hanghou 510029 (China)
2 The Second Research Institute of Oceanography, Hagzhon 510029 (China)
      Nine soils with distinct properties and Se levels were selected to test a fractionation procedure of soil Se based on sequential extraction. Soil Se was fractionated into readily available Se (fraction Ⅰ, extracted by 0.5 M NaHCO3), slowly available Se (fraction Ⅱ, extracted by 0.1 M NaOH-0.1 M Na4P2O7), amorphous oxide-occluded Se (fraction Ⅲ, extracted by acid ammonium oxalate), free oxide-occluded Se (fraction Ⅵ, extracted by dithionite-citrate-bicarbonate buffer solution) and residual Se (fraction Ⅴ, determined by HNO3-HClO4 digestion of the final soil residue). The recovery of soil Se (the sum of all fractions over total soil Se determined independently) by this procedure was from 88.1% to 110.9%, mean 99.2%±6.4% for the test soils. The sum of fractions Ⅰ and Ⅱ, provided a good measure of available Se in soils and the percentage of fraction Ⅰ plus Ⅱ over the total soil Se, tentatively defined as Se availability index, could be used to indicate soil Se status and predict Se deficiency.
Key Words:  availability, fractionation, selenium, soil
Citation: He, Z. L., YANG Xiao-E, Zhu, Z. X., Xia, W. P., Pan, J. M. and Liu, X. Y. 1996. Fractionation of soil selenium with relation to Se availability to plants. Pedosphere. 6(3): 209-216.
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