Pedosphere 4(3): 201--208, 1994
ISSN 1002-0160/CN 32-1315/P
©1994 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Effect of acetylene concentration on denitriflcation and related microbial processes in anaerobically incubated soil slurries
CHEN Tong-Bin1, S. STRUWE2 and A. KJΦLLER2
1 Experimental Station for Agruecology and Environmental Technology, Institute of Geogmphy, Chtnese Academy of Sctences, Beijing 100101 (China)
2 Department of General Microbiology, University of Copenhagen, SφIvgade 83 H, DK-1307 Copenhagen K (Denmark)
ABSTRACT
      In order to study the influence of difference in C2H2 concentration on the production of CO2, N2O, NH4-N and volatile fatty acids (VFA), soil slurries with a gradient in C2H2 concentration were anaerobically incubated at 25℃ for 2 weeks. Acetate, butyrate and CO2 production and NH4-N accumulation were inhibited in the slurries in the presence of C2H2; and the inhibition effect increased with increasing C2H2 from 0 to 20 kPa in the headspace gas of the incubation bottle. However, N2O, isobutyrate and propionate production was not obviously different among the slurries amended with C2H2 from 2.5 to 20 kPa. Therefore, the results implied that the C2H2 did not promote the inhibition but only increased the side effect on other microbial processes. The C2H2 of 2.5 kPa was suggested to be the optimum choice for the present denitrification study.
Key Words:  acetylene, anaerobic incubation, carbon dioxide, nitrogen transformation, volatile fatty acids
Citation: Chen, T. B., Struwe, S. and Kjφller, A. 1994. Effect of acetylene concentration on denitriflcation and related microbial processes in anaerobically incubated soil slurries. Pedosphere. 4(3): 201-208.
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