Pedosphere 3(2): 127--132, 1993
ISSN 1002-0160/CN 32-1315/P
©1993 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Kinetic Characteristics of Nitrate Uptake by Wheat and Rape and Their Determination |
ZHANG LI-GAN, ZHU WEI-MIN, XUAN JIA-XIANG |
Anhui Agricultural College, Hefei 230036 |
ABSTRACT |
15-day old seedlings of wheat and rape were grown in a series of solutions with different concentrations of KNO3 for a definite period of time. The changes in NO3- concentration of the solutions were determined by the double ion-selective electrode method, and then the amount of NO3- taken up by the plants was estimated and values of Km and Imax of the Michealis-Menten equation were calculated. Results show that both the method and conditions of determination affected the values of Km and Imax. For example, the Km value was appreciably reduced when the volume of culture solution was increased or when the duration of nutrient uptake was shortened; the Km value obtained with short-term depletion method was higher than that obtained with long-term one. Similar Variations were found for the values of Imax. |
Key Words: |
Citation: Zhang, L. G., Zhu, W. M. and Xuan, J. X. 1993. Kinetic Characteristics of Nitrate Uptake by Wheat and Rape and Their Determination. Pedosphere. 3(2): 127-132. |
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