Elsevier

Pedosphere

Volume 30, Issue 3, June 2020, Pages 405-413
Pedosphere

Soil organic carbon and phosphorus availability regulate abundance of culturable phosphate-solubilizing bacteria in paddy fields

https://doi.org/10.1016/S1002-0160(17)60403-XGet rights and content

Abstract

Low availability of phosphorus (P) is a major constraint for optimal crop production, as P is mostly present in its insoluble form in soil. Therefore, phosphate-solubilizing bacteria (PSB) from paddy field soils of the Indo-Gangetic Plain, India were isolated, and their abundance was attempted to be correlated with the physicochemical characteristics of the soils. Ninety-four PSB were isolated on Pikovskaya's agar medium, and quantitative phosphate solubilization was evaluated using NBRIP medium. The isolates solubilized P up to a concentration of 1 006 μg mL−1 from tricalcium phosphate with the secretion of organic acids. These isolates were identified by 16S rRNA gene sequence comparison, and they belonged to Gammaproteobacteria (56 isolates), Firmicutes (28 isolates), Actinobacteria (8 isolates), and Alphaproteobacteria (2 isolates). Phylogenetic analysis confirmed the identification by clustering the isolates in the clade of the respective reference organisms. The correlation analysis between PSB abundance and physicochemical characteristics revealed that the PSB population increased with increasing levels of soil organic carbon, insoluble P, K+, and Mg2+. The promising PSB explored in this study can be further evaluated for their biofertilizer potential in the field and for their use as potent bio-inoculants.

References (50)

  • T F Lin et al.

    The protons of gluconic acid are the major factor responsible for the dissolution of tricalcium phosphate by Burkholderia cepacia CC-Al74

    Bioresour Technol

    (2006)
  • J C López-Gutiérrez et al.

    Seasonality of organic phosphorus mineralization in the rhizosphere of the native savanna grass, Trachypogon plumosus

    Soil Biol Biochem

    (2004)
  • C S Nautiyal

    An efficient microbiological growth medium for screening phosphate solubilizing microorganisms

    FEMS Microbiol Lett

    (1999)
  • C S Nautiyal et al.

    Stress induced phosphate solubilization in bacteria isolated from alkaline soils

    FEMS Microbiol Lett

    (2000)
  • E Pérez et al.

    Isolation and characterization of mineral phosphate-solubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region

    Soil Biol Biochem

    (2007)
  • D Pezzolla et al.

    Influence of exogenous organic matter on prokaryotic and eukaryotic microbiota in an agricultural soil. A multidisciplinary approach

    Soil Biol Biochem

    (2015)
  • H Rodríguez et al.

    Phosphate solubilizing bacteria and their role in plant growth promotion

    Biotechnol Adv

    (1999)
  • Y P Wu et al.

    Organic amendment application influence soil organism abundance in saline alkali soil

    Eur J Soil Biol

    (2013)
  • H Yadav et al.

    Optimization of culture conditions for phosphate solubilization by a thermo-tolerant phosphate-solubilizing bacterium Brevibacillus sp. BISR-HY65 isolated from phosphate mines

    Biocatal Agric Biotechnol

    (2013)
  • S Aarab et al.

    Isolation and screening of inorganic phosphate solubilizing Pseudomonas strains from rice rhizosphere soil from Northwestern Morocco

    Amer J Res Commun

    (2015)
  • K J Chin et al.

    Characterization and identification of numerically abundant culturable bacteria from the anoxic bulk soil of rice paddy microcosms

    Appl Environ Microbiol

    (1999)
  • A D Eaton et al.

    Standard Methods for the Examination of Water and Waste Water

    (1995)
  • A H Goldstein et al.

    Molecular cloning and regulation of a mineral phosphate solubilizing gene from Erwinia herbicola

    Bio/Technology

    (1987)
  • A Gulati et al.

    Characterization of phosphate-solubilizing fluorescent pseudomonads from the rhizosphere of Seabuckthorn growing in the cold deserts of Himalayas

    Curr Microbiol

    (2008)
  • J M Igual et al.

    Phosphate-solubilizing bacteria as inoculants for agriculture: Use of updated molecular techniques in their study

    Agronomie

    (2001)
  • Cited by (20)

    • Physiological regulations of a highly tolerant cactus to dry season modify its rhizospheric microbial communities

      2023, Rhizosphere
      Citation Excerpt :

      However, only some studies directly and specifically correlate amino acid exudation with the abundance of the nifH gene (Ando et al., 2005; Zhou et al., 2021). Secondly, the availability of carbon under the plant can stimulate the activity of bacteria mineralizing phosphorus (Kumar and Rai, 2020). Therefore, the phoD gene copies of the organic P mineralizers bacteria were higher in the rhizosphere than in the bulk soil.

    • Electroactive constructed wetland using Fe<inf>3</inf>C as an anodic exogenous electron donor: Performance and mechanisms

      2022, Journal of Water Process Engineering
      Citation Excerpt :

      Gammaproteobacteria and Alphaproteobacteria are vital EAB and play an important role in biogeochemical cycles. Gammaproteobacteria may be responsible for phosphorus removal [48], and they could also contribute to the high denitrification and dephosphorization efficiencies observed in the two CW-MFC configurations. Gammaproteobacteria can transfer electrons from the cytochrome to the outer membrane and the cell wall to improve electrical performance in a MFC [49].

    • Reclamation of waste coal gangue activated by Stenotrophomonas maltophilia for mine soil improvement: Solubilizing behavior of bacteria on nutrient elements

      2022, Journal of Environmental Management
      Citation Excerpt :

      Currently, in agricultural practice, farmers use excess amounts of chemical fertilizers to the soil each year for plant growth (Chen et al., 2006, 2008). Unfortunately, 80% of the available P in soluble inorganic fertilizers is rapidly precipitated and unavailable by plants (Khan et al., 2010; Kumar et al., 2020). In China, about 95% of P in arable land is immobile and cannot be absorbed by plants (Zhang et al., 2020).

    • Improvement of phosphorus uptake, phosphorus use efficiency, and grain yield of upland rice (Oryza sativa L.) in response to phosphate-solubilizing bacteria blended with phosphorus fertilizer

      2022, Pedosphere
      Citation Excerpt :

      Soil microbial count was found to be maximum in the PSB treatment with 50% P fertilizer. Soil available P and biofertilizer application in seeds, soil, and roots are factors that affect microbial abundance in soil (Kumar and Rai, 2020). Thus, P fertilizer is an active metabolic substrate for soil microorganisms, and its addition to soil activates soil microbial activity and growth (Bhatt et al., 2016).

    View all citing articles on Scopus
    View full text