Use of High-Yielding Bioenergy Plant Castor Bean (Ricinus communis L.) as a Potential Phytoremediator for Copper-Contaminated Soils

doi:10.1016/S1002-0160(13)60057-0

Pedosphere

Volume 23, Issue 5, October 2013, Pages 651-661

https://doi.org/10.1016/S1002-0160(13)60057-0 Get rights and content

Abstract

Copper (Cu) contamination in the environment has been increased during the years with agricultural and industrial activities. Biotechnological approaches are needed for bioremediation in these areas. The aims of this study were i) to evaluate the phytoremediation capacity of the high-yielding bioenergy plant castor bean (Ricinus communis L.) in vineyard soils (Inceptisol and Mollisol) contaminated with Cu and a Cu mining waste; ii) to characterize the castor bean as a Cu phytoremediation plant; and iii) to evaluate the nutrient uptake by castor bean. Castor bean plants cultivated in soil with toxic levels of Cu for 57 d exhibited high phytomass production, a high tolerance index of roots' fresh mass and shoots' dry mass, a high level of Cu phytoaccumulation in the roots and also, a robust capacity for Cu phytostabilization. Furthermore, castor bean plants did not significantly deplete soil nutrients (such as N, P, and Mg) during cultivation. Plants cultivated in Inceptisol, Mollisol and Cu mining waste exhibited a strong potential for Cu phytoaccumulation, with values of 5 900, 3 052 and 2 805 g ha⁻¹, respectively. In addition, the castor bean's elevated phytomass production and strong growth in Cu-contaminated soils indicated a high level of Cu phytoaccumulation and a potential application in biofuels. These findings indicate that the castor bean is a efficient hyperaccumulator of Cu and a potential candidate plant for the phytoremediation of Cu-contaminated soil.

References (32)

P. Andráš et al.
Hazardous of heavy metal contamination at L'ubietová Cu-deposit (Slovakia)
Proced. Environ. Sci.
(2012)
R. Andreazza et al.
Bacterial stimulation of copper phytoaccumulation by bioaugmentation with rhizosphere bacteria
Chemosphere.
(2010)
R. Andreazza et al.
Characterization of copper bioreduction and biosorption by a highly copper resistant bacterium isolated from copper-contaminated vineyard soil
Sci. Total Environ.
(2010)
D. Champier et al.
Thermoelectric power generation from biomass cook stoves
Energy.
(2010)
R. Clemente et al.
A remediation strategy based on active phytoremediation followed by natural attenuation in a soil contaminated by pyrite waste
Environ. Pollut.
(2006)
G. García et al.
Performance of Piptatherum miliaceum (Smilo grass) in edaphic Pb and Zn phytoremediation over a short growth period
Int. Biodeter. Biodegr.
(2004)
V.N. Kavamura et al.
Biotechnological strategies applied to the decontamination of soils polluted with heavy metals
Biotechnol. Adv.
(2010)
H. Lequeux et al.
Response to copper excess in Arabidopsis thaliana: Impact on the root system architecture, hormone distribution, lignin accumulation and mineral profile
Plant Physiol. Biochem.
(2010)
C. Mant et al.
Phytoremediation of chromium by model constructed wetland
Bioresource Technol.
(2006)
E.E.C. Melo et al.
Accumulation of arsenic and nutrients by castor bean plants grown on an As-enriched nutrient solution
J. Hazard. Mater.
(2009)

J. Mertens et al.

Use and abuse of trace metal concentrations in plant tissue for biomonitoring and phytoextraction

Environ. Pollut.

(2005)

B. Nedjimi et al.

Cadmium accumulation in Atriplex halimus subsp. schweinfurthii and its influence on growth, proline, root hydraulic conductivity and nutrient uptake

Flora.

(2009)

D.S. Ogunniyi

Castor oil: A vital industrial raw material

Bioresource Technol.

(2006)

J.R. Peralta-Videa et al.

Effect of mixed cadmium, copper, nickel and zinc at different pHs upon alfalfa growth and heavy metal uptake

Environ. Pollut.

(2002)

F.S. Santos et al.

Chelateinduced phytoextraction of metal polluted soils with Brachiaria decumbens

Chemosphere.

(2006)

V. Scholz et al.

Prospects and risks of the use of castor oil as a fuel

Biomass Bioenerg.

(2008)

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: Supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) of Brazil and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) of Brazil.

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Use of High-Yielding Bioenergy Plant Castor Bean (Ricinus communis L.) as a Potential Phytoremediator for Copper-Contaminated Soils

Abstract

Proced. Environ. Sci.

Chemosphere.

Sci. Total Environ.

Energy.

Environ. Pollut.

Int. Biodeter. Biodegr.

Biotechnol. Adv.

Plant Physiol. Biochem.

Bioresource Technol.

J. Hazard. Mater.

Environ. Pollut.

Flora.

Bioresource Technol.

Environ. Pollut.

Chemosphere.

Biomass Bioenerg.