Bacterial Community Structure in a Mollisol Under Long-Term Natural Restoration, Cropping, and Bare Fallow History Estimated by PCR-DGGE*1
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Cited by (21)
Effects of short-term fallow managements on soil microbial properties: A case study in China
2018, Applied Soil EcologyCitation Excerpt :Hence, α-diversities were relatively improved by fertilizer and farmyard manure in the incipient stage (spring), which presented obvious differences, whereas they finally turned into no discernible difference in the autumn. We also found that the CK experienced an obvious diversity drop during the second stage (summer), whereas native grass showed advantages, which is consistent with some studies on natural restoration (Allen et al., 2001; Wang et al., 2009). In combining the α-diversity, Venn (Fig. A3) and dissimilarity analyses, we inferred that (i) autumn is a relatively steady stage that reliably reflected the changes in bacteria; (ii) in the absence of fertilizer management, the participation of pastures presented fewer fluctuations during the year; and (iii) the CK could experience a remarkable fall in microbial diversity after fertilization.
Effects of an underground in situ pipe eco-farming system on mangrove bacterial communities in the Beilunhe Estuary, Guangxi, China
2014, Ecological EngineeringCitation Excerpt :Normal sequences were compared with reference sequences in Genebank using BLAST, the operational taxonomic units (OTUs) were determined using DNAMAN at a sequence similarity level of >97%. All 16S rDNA sequences were fitted into a phylogenetic tree (neighbor-joining method) using MEGA 4.0 (Wang et al., 2009). TBA (the data were log10-transformed prior to comparison) and community indices of bacteria in seawater collected from the underground in situ pipes, the natural mangroves and the reservoir were taken into estimating the horizontal differences in an one-way analysis of variance (ANOVA, LSD) performed in SPSS 17.0, which was also used to analyze the variations of these parameters among different sampling events.
Soil microbial communities are affected more by land use than seasonal variation in restored grassland and cultivated Mollisols in Northeast China
2011, European Journal of Soil BiologyCitation Excerpt :Therefore, the bands excised from a DGGE gel in this study were considered as the dominating microbial members in the studied black soil. Although the number of bands excised for sequencing was limited, the results of the BLAST search suggested that bacteria belonging to Proteobacteria and Actinobacteria, and fungi of Ascomycota and Basidiomycota phyla were the dominant microbial populations in the black soil, which concurred with previous reports in Northeast China [40,41]. Although the DGGE method used in this study has its intrinsic limitation to elucidate only the dominant members in the microbial communities, the present study found the difference in microbial communities between restored grassland and cultivated soils and seasonal variations of the communities in black soil in Northeast China.
A Mechanistic Model of Microbially Mediated Soil Biogeochemical Processes: A Reality Check
2019, Global Biogeochemical Cycles
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Project supported by the National Natural Science Foundation of China (No. 40671099).