Elsevier

Pedosphere

Volume 18, Issue 6, December 2008, Pages 801-808
Pedosphere

Effect of Long-Term Application of Chemical Fertilizers on Microbial Biomass and Functional Diversity of a Black Soil*1

https://doi.org/10.1016/S1002-0160(08)60076-4Get rights and content

Abstract

An experiment with seven N, P, K-fertilizer treatments, i.e., control (no fertilizer), NP, NK, PK, NPK, NP2K, and NPK2 where P2 and K2 indicate double amounts of P and K fertilizers respectively, was conducted to examine the effect of long-term continuous application of chemical fertilizers on microbial biomass and functional diversity of a black soil (Udoll in the USDA Soil Taxonomy) in Northeast China. The soil microbial biomass C ranged between 94 and 145 mg kg−1, with the NK treatment showing a lower biomass; the functional diversity of soil microbial community ranged from 4.13 to 4.25, with an increasing tendency from control to double-fertilizer treatments, and to triple-fertilizer treatments. The soil microbial biomass, and the microbial functional diversity and evenness did not show any significant differences among the different fertilizer treatments including control, suggesting that the long-term application of chemical fertilization would not result in significant changes in the microbial characteristics of the black soil.

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    *1

    Project supported by the National Natural Science Foundation of China (No. 40321101), the Ministry of Science and Technology of China (No. 2005CB121105) and the Knowledge Innovation Program of the Chinese Academy of Sciences (Nos. KZCX1-SW-19 and KZCX2-YW-408).

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