Bacterial Community Structure and Diversity in a Black Soil as Affected by Long-Term Fertilization

doi:10.1016/S1002-0160(08)60052-1

Pedosphere

Volume 18, Issue 5, October 2008, Pages 582-592

https://doi.org/10.1016/S1002-0160(08)60052-1 Get rights and content

Abstract

Black soil (Mollisol) is one of the main soil types in northeastern China. Biolog and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) methods were used to examine the influence of various fertilizer combinations on the structure and function of the bacterial community in a black soil collected from Harbin, Heilongjiang Province. Biolog results showed that substrate richness and catabolic diversity of the soil bacterial community were the greatest in the chemical fertilizer and chemical fertilizer+manure treatments. The metabolic ability of the bacterial community in the manure treatment was similar to the control. DGGE fingerprinting indicated similarity in the distribution of most 16S rDNA bands among all treatments, suggesting that microorganisms with those bands were stable and not influenced by fertilization. However, chemical fertilizer increased the diversity of soil bacterial community. Principal component analysis of Biolog and DGGE data revealed that the structure and function of the bacterial community were similar in the control and manure treatments, suggesting that the application of manure increased the soil microbial population, but had no effect on the bacterial community structure. Catabolic function was similar in the chemical fertilizer and chemical fertilizer+manure treatments, but the composition structure of the soil microbes differed between them. The use of chemical fertilizers could result in a decline in the catabolic activity of fast-growing or eutrophic bacteria.

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^*1: Project supported by the Heilongjiang Provincial Natural Science Funds for Distinguished Young Scholars, China (No. JC200622), the Heilongjiang Provincial Natural Science Foundation of China (No. C2004-17), and the National “Eleventh Five Years Plan” Key Project on Science and Technology of China (No. 2006BAD25B05).

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Bacterial Community Structure and Diversity in a Black Soil as Affected by Long-Term Fertilization*1

Abstract

Soil Biol. Biochem.

J. Microbiol. Meth.

FEMS Microbiol. Ecol.

Geoderma.

J. Microbiol. Meth.

J. Microbiol. Meth.

Soil Biol. Biochem.

Chemosphere.

Soil Biol. Biochem.

Soil Biol. Biochem.

The Health of Our Soils: Toward Sustainable Agriculture in Canada

Separation and purification of bacteria from soil

Appl. Environ. Microb.

Determinants of soil microbial communities: Effects of agricultural management, season and soil type on phospholipid fatty acid profiles

Microbial Ecol.

Determination of total, organic, and available forms of phosphorus in soils

Soil Sci.

The use of microbial parameters in monitoring soil pollution by heavy metals

Biol. Fertil. Soils.

Succession and phylogenetic composition of bacterial communities responsible for the composting process of rice straw estimated by PCR-DGGE analysis

Soil Sci. Plant Nutr.

Challenges related to soil biodiversity research in agroecosystems-Issues within the context of scale of observation

Can. J. Soil Sci.

Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization

Appl. Environ. Microb.

Biological attributes of soil quality

Bacterial Community Structure and Diversity in a Black Soil as Affected by Long-Term Fertilization^*1