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土壤圈(英文版). 1999, (02)

Determination of Organic Acids in Root Exudates byHigh Performance Liquid Chromatography: III. Effectsof Interfering FactorsEnglish Full Text

SHEN JIANBO; ZHANG FUSUO and MAO DARU(Department of Plant Nutrition, China Agricultural University, Beijing 100094 (China)

Abstract: A solution culture experiment was conducted to investigate the effects of collection time and interferingions on separation and determination of low-molecular-weight organic acids in root exudates of soybeanusing the method for directly collecting root exudates. The suitable collection time of root exudates andthe interfering ions affecting organic acid determination were determined. The method for removing theinterfering ions was established and analyzed. The release amount of root exudates increased with theincrease of collection time from 0 to 120 min but decreased with increasing of collection time from 120 to 240min. The maximum exuding amounts of organic acids were observed in root exudates at the collection time of120 min. There was a significant difference of organic acid components between the treatments of collectiontime of 120 min and 240 min. Citric acid was found only in the treatment of 120 min collection time. NO3-was the main interfering ion in organic acid determination and had the same retention time as oxalic acid.Anion exchangs resin （SAX） properly treated by HPLC （high performance liquid chromatography） solventcould remove NO3- anion in sample solution of root exudates, thus enhancing the recoveries of organic acidsin root exudates. There was no significant effect of the chemicals added into sample solution such as H3PO4,SAX and KNO3 on the retention time of organic acids.
Keywords：

collection time; interfering factor; low-molecular-weight organic acid; root exudates;

  • Series：

    (D) Agriculture

  • Subject：

    Agronomy; Crop

  • Classification Code：

    S311

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