Pedosphere 35(6): 1016--1025, 2025
ISSN 1002-0160/CN 32-1315/P
©2025 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Dynamic responses of endophytic microbial communities of Jerusalem artichoke to the absence of nitrogen fertilizer |
| Mengjia ZHOU1,2,3, Ji WANG1,2,3, Ruixuan YANG1,3, Qian CUI1,3, Xin XU1,3, Jingjing XU1, Huijuan ZHANG1, Ebru TOKSOY4, NER1,3 |
1 College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095 (China);
2 School of Geography and Planning, Chizhou University, Chizhou 247000 (China);
3 Jiangsu Provincial Key Laboratory of Coastal Saline Soil Resources Utilization and Ecological Conservation, Nanjing Agricultural University, Nanjing 210095 (China);
4 IBSB, Department of Bioengineering, Faculty of Engineering, Marmara University, Istanbul 34722 (Turkey) |
| ABSTRACT |
| The taxonomic composition and function of endophytic microbial communities in plants are strongly influenced by environmental changes. The impact of nitrogen (N) fertilization on the diversity of microbial communities in the underground parts of plants that are tolerant of barren environments throughout their growth period remains largely unexplored. Here, by high-throughput sequencing technology, an experiment was performed at the Pailou Experimental Station of Nanjing Agricultural University, where the soil is a yellow-brown soil, to analyze fluctuations in the microbial communities of Jerusalem artichoke (Helianthus tuberosus L.) underground parts in two treatments, application of N, P, and K fertilizers (NPK) and application of only P and K fertilizers (PK), from March 2018 to January 2019. Total, nitrate, and ammonium N contents of soil and Jerusalem artichoke were significantly decreased in the PK treatment. The bacterial community, particularly Acinetobacter, dominated throughout the growth period, peaking in November and accounting for 88.9% and 87.3%, respectively, in the NPK and PK treatments. The absence of N fertilizer (i.e., NP treatment) had minimal effect on the bacterial community composition compared to the fungal community composition. The α-diversity of bacteria in tubers was not substantial, while that in roots was dramatically reduced in July and September in the NP treatment. Notably, the α-diversity of fungal species exhibited more pronounced seasonal variations than that of bacteria under both fertilizer conditions. The lack of N fertilizer lessened the complexity of bacterial network structure and reduced bacterial community similarity (β-diversity) in different months. Interestingly, the β-diversity and network structure of fungal community in the underground tissues of Jerusalem artichoke showed less sensitivity to N fertilizer compared to those of bacterial community. This study improves the understanding of the dynamic response of endophytic microorganisms to N fertilizer application in the underground parts of Jerusalem artichoke tolerant to barren environments. |
| Key Words: community composition|high-throughput sequencing|microbial diversity|network structure|underground part |
| Citation: Zhou M J, Wang J, Yang R X, Cui Q, Xu X, Xu J J, Zhang H J, Toksoy Öner E, Liang M X. 2025. Dynamic responses of endophytic microbial communities of Jerusalem artichoke to the absence of nitrogen fertilizer. Pedosphere. 35(6): 1016-1025. |
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