欢迎访问PEDOSPHERE网站！

Pedosphere (3): 580--590, 2025
ISSN 1002-0160/CN 32-1315/P
©2025 Soil Science Society of China
Published by Elsevier B.V. and Science Press

Elevated CO₂ enhances glomalin accumulation and cadmium (Cd) sequestration in soil of Funneliformis mosseae-inoculated black locust exposed to Cd contamination

Xiaojuan FENG¹, Xia JIA¹

, Yonghua ZHAO², Xuelian YANG¹, Liangyu HUANG¹, Chunyan ZHANG¹, Yunfeng GAO²

¹ Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region of Ministry of Education, Shaanxi Key Laboratory of Land Consolidation, Key Laboratory of Eco-hydrology and Water Security in Arid and Semi-arid Regions of Ministry of Water Resources, School of Water and Environment, Chang'an University, Xi'an 710054 (China);
² School of Land Engineering, Chang'an University, Xi'an 710054 (China)

ABSTRACT

Glomalin, an extensively secreted glycoprotein produced by arbuscular mycorrhizal fungi (AMF), plays a crucial role in heavy metal sequestration, though its functionality is highly susceptible to various environmental factors. Due to the limitations in purification techniques, glomalin is often assessed indirectly as glomalin-related soil proteins (GRSP). Although the effect of elevated carbon dioxide (eCO₂) on GRSP under heavy metal stress has been widely investigated, the quantitative contribution of eCO₂ to GRSP is still unclear. Here, we employed a ¹³CO₂ isotopic labeling approach to assess the contribution of eCO₂ (285 μL L^-1 above ambient CO₂) to GRSP in the bulk and rhizosphere soils of black locust seedlings colonized by Funneliformis mosseae under cadmium (Cd) exposure. The contribution of eCO₂ to easily extractable GRSP (EE-GRSP) in the rhizosphere soil was 8.3 μg g^-1 soil under F. mosseae colonization. The contribution of eCO₂ to total GRSP (T-GRSP) in the rhizosphere soil reached 26.1 μg g^-1 soil under F. mosseae colonization. However, eCO₂ showed negative contribution to EE-GRSP in the bulk soil, and its contribution to T-GRSP in the bulk soil under F. mosseae colonization was 7.6 μg g^-1 soil. Additionally, the highest contribution of eCO₂ to Cd sequestration in the rhizosphere soil was 0.93 mg kg^-1 soil under F. mosseae colonization. The results suggest that F. mosseae enhances Cd sequestration in soil through glomalin release, highlighting its potential role in heavy metal stabilization under eCO₂ conditions in AMF-colonized soils.

Citation: Feng X J, Jia X, Zhao Y H, Yang X L, Huang L Y, Zhang C Y, Gao Y F. 2025. Elevated CO₂ enhances glomalin accumulation and cadmium (Cd) sequestration in soil of Funneliformis mosseae-inoculated black locust exposed to Cd contamination. Pedosphere. 35(3): 580-590.

View Full Text