Pedosphere 30(5): 618--627, 2020
ISSN 1002-0160/CN 32-1315/P
©2020 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Optimization and modeling of glyphosate biodegradation by a novel Comamonas odontotermitis P2 through response surface methodology |
Sadiqa FIRDOUS1,2, Samina IQBAL1,2 , Samina ANWAR1 |
1Soil and Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad 38000 (Pakistan);
2Pakistan Institute of Engineering and Applied Sciences (PIEAS), Nilore, Islamabad 45650 (Pakistan) |
| ABSTRACT |
| Glyphosate is an important organophosphonate herbicide used to eliminate grasses and herbaceous plants in many vegetation management situations. Its extensive use is causing environmental pollution, and consequently, there is a need to remove it from the environment using an eco-friendly and cost-effective method. As a step to address this problem, a novel bacterial strain Comamonas odontotermitis P2, capable to utilize glyphosate as a carbon (C) and/or phosphorus (P) source, was isolated from a glyphostate-contaminated field soil in Australia and characterized. Response surface methodology (RSM) employing a 23 full factorial central composite design was used to optimize glyphosate degradation by C. odontotermitis P2 under various culture conditions. The strain C. odontotermitis P2 was proficient in degrading 1.5 g L-1 glyphosate completely within 104 h. The optimal conditions for the degradation of glyphosate were found to be pH 7.4, 29.9℃, and an inoculum density of 0.54 g L-1, resulting in a maximum degradation of 90%. Sequencing of glyphosate oxidoreductase (GOX) and C-P lyase (phnJ) genes from C. odontotermitis P2 revealed 99% and 93% identities to already reported bacterial GOX and phnJ genes, respectively. The presence of these two genes in C. odontotermitis indicates its potential to degrade glyphosate through GOX and C-P lyase metabolic pathways. This study demonstrates the potential of C. odontotermitis P2 for efficient degradation of glyphosate, which can be exploited for remediation of glyphosate. |
| Key Words: bacterial strain,central composite rotatable design,glyphosate oxidoreductase,high-performance liquid chromatography,inoculum density,organophosphonate herbicide,remediation |
| Citation: Firdous S, Iqbal S, Anwar S. 2020. Optimization and modeling of glyphosate biodegradation by a novel Comamonas odontotermitis P2 through response surface methodology. Pedosphere. 30(5):618-627. |
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