Pedosphere 30(2): 285--292, 2020
ISSN 1002-0160/CN 32-1315/P
©2020 Soil Science Society of China
Published by Elsevier B.V. and Science Press
Effect of environmental C/N ratio on activities of lignin-degrading enzymes produced by Phanerochaete chrysosporium |
Saihua HUANG3, Deyin HUANG3, Qitang WU1, Meifang HOU2,3, Xiaoyan TANG2,3, Jian ZHOU2 |
1College of Natural Resources and Environment, South China Agricultural University, 483 Wushan Street, Guangzhou 510642 (China); 2School of Ecological Technology and Engineering, Shanghai Institute of Technology, 100 Haiquan, Shanghai 201418 (China); 3Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-environmental and Soil Sciences, 808 Tianyuan Road, Guangzhou 510650 (China) |
Corresponding Author:Saihua HUANG |
ABSTRACT |
Lignin-degrading enzymes secreted by white rot fungi play an important role in the degradation of lignin and persistent organic pollutants (POPs). In this study, effect of environmental C/N ratio on the activities of lignin-degrading enzymes, lignin peroxide (LiP) and manganese peroxidase (MnP), produced by Phanerochaete chrysosporium, a white rot fungus, was investigated. Glucose was used as C source, and ammonium tartrate of different concentrations was used as N source to provide different C/N ratios. Relationships between LiP and MnP activities and environmental C/N ratio were explored. The results showed that the higher the N source concentration, the faster the mycelium pellets aged. The faster the mycelium dry weight increased, the higher the LiP and MnP activities. A high C/N ratio was a necessary condition for the secretion of LiP or MnP. In addition, mycelium dry weight essentially affected enzyme activities. In the 122 C/N ratio and 50 C/N ratio treatments, mycelium dry weight essentially affected MnP activity and both LiP and MnP activities, respectively. |
Key Words: biodegration,lignin peroxide,manganese peroxidase,peroxidase,persistent organic pollutant,polysaccharide,white rot fungus |
Citation: Huang S H, Huang D Y, Wu Q T, Hou M F, Tang X Y, Zhou J. 2020. Effect of environmental C/N ratio on activities of lignin-degrading enzymes produced by Phanerochaete chrysosporium. Pedosphere. 30(2): 285-292. |
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