Pedosphere 26(5): 768--773, 2016
ISSN 1002-0160/CN 32-1315/P
©2016 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| A modified plate assay for rapid screening of potassium-solubilizing bacteria |
M. V. S. RAJAWAT, S. SINGH, S. P. TYAGI and A. K. SAXENA |
| Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012 (India) |
| ABSTRACT |
| The utility of microorganisms for solubilizing the unavailable forms of potassium (K) from soil has led to renewed interest in fabrication of rapid and sensitive plate assays for their isolation and screening. The present study developed a modified plate assay and compared it with previously reported methods for the isolation and screening of K-solubilizing bacteria. The newly developed plate assay is based on improved visualization of halo zone formation around the colonies on agar plates, through inclusion of an acid-base indicator dye, bromothymol blue (BTB), to modify the previously reported Aleksandrov medium. The halo zone exhibited a significant correlation (R = 0.939) with K released in liquid medium. The visualization of potential K solubilizers was improved using this method, which would help in detection of weak/non-acid producers based on secretion of organic acids in the medium. Organic acids in plate diffuse radially and form halo zones in response to reaction with the acid-base indicator dye BTB. Furthermore, K solubilization on plates with this method can be observed within 48-72 h, against the incubation time of 4-5 d needed in the earlier method. Therefore, the newly developed protocol for the plate assay was time saving, more sensitive, and beneficial in comparison to the previously reported Aleksandrov plate assay. |
| Key Words: bromothymol blue (BTB), Aleksandrov medium, halo zone formation, indicator dye, K solubilizers, microorganisms |
| Citation: Rajawat, V. S., Singh, S., Tyagi, S. P. and Saxena, A. K. 2016. A modified plate assay for rapid screening of potassium-solubilizing bacteria. Pedosphere. 26(5): 768-773. |
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