Pedosphere 23(3): 350--358, 2013
ISSN 1002-0160/CN 32-1315/P
©2013 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Effects of soil depth and season variation on community structure of arbuscular mycorrhizal fungi in greenhouse soils planted with watermelon |
LIU Run-Jin1, LI Yan1, DIAO Zhi-Kai1, LI Min1 and LIN Xian-Gui2 |
1 Institute of Mycorrhizal Biotechnology, Qingdao Agricultural University, Qingdao 266109 (China)
2State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China) |
| ABSTRACT |
| The characteristics of arbuscular mycorrhizal fungal (AMF) community structure in various soil depths and growing seasons of watermelon (Citrullus vulgaris) grown in commercial greenhouses in Daxing of Beijing and Weifang and Laiyang of Shandong, China were investigated using both morphological identification and denaturing gradient gel electrophoresis. The sampled soils had been used for continuous greenhouse production of watermelon for 0, 5, 10, 15, or 20 years. Glomus claroideum was the dominant species in the greenhouse soil planted for 5, 10, and 15 years in Laiyang, while Glomus mosseae and Glomus etunicatum were dominant in the nearby open farmland soil. Sorenson’s similarity indices of AMF community composition ranged from 0.67 to 0.84 in the soils planted for 5 years, and from 0.29 to 0.33 for 20 years among the three locations. Spore abundance, species richness, and the Shannon index were highest near the soil surface (0--10 cm) and decreased with soil depth, and higher in June and October than in August and December. Canonical correspondence analysis showed that available P and the number of years that soil had been used for in greenhouse production were the main factors contributing to the variance of AMF community composition. It was concluded that the community structure of AMF was mainly influenced by soil available P and culturing time of watermelon as well as by soil depth and seasonal variation in the commercial greenhouse. |
| Key Words: canonical correspondence analysis, Shannon index, Sorenson’s similarity indices, species richness, spore abundance |
| Citation: Liu, R. J., Li, Y., Diao, Z. K., Li, M. and Lin, X. G. 2013. Effects of soil depth and season variation on community structure of arbuscular mycorrhizal fungi in greenhouse soils planted with watermelon. Pedosphere. 23(3): 350-358. |
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