Pedosphere 22(4): 554--561, 2012
ISSN 1002-0160/CN 32-1315/P
©2012 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Biodegradation of benzo[a]pyrene by Arthrobacter oxydans B4 |
PENG Hui1,2, YIN Hua1,2 , DENG Jun1,2, YE Jin-Shao1,2, CHEN Shuo-Na1,2, HE Bao-Yan1,2 and ZHANG Na1,2 |
1 Department of Environmental Engineering, Jinan University, Guangzhou 510632 (China);
2 Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632 (China) |
| ABSTRACT |
| A bacterial strain, Arthrobacter oxydans (B4), capable of degrading benzo [a] pyrene (BaP) in water body, was isolated from a polycyclic aromatic hydrocarbons-contaminated site. Effects of different factors, such as reaction time, pH value, temperature and organic nutrients, on BaP biodegradation by the strain B4 were studied. After 5 d treatment, the concentration of BaP in mineral salts medium was reduced to 0.318 mg L-1, compared to the initial concentration of 1.000 mg L-1. There was a process of acid formation during the degradation with pH falling from initial 7.01 to 4.61 at 5 d, so keeping the water body under slightly alkaline condition was propitious to BaP degradation. Strain B4 efficiently degraded BaP at 20 to 37 ℃ with addition of organic nutrients. The biodegradation and transformation of Ba P mainly occurred on cell surfaces, and extracellular secretions played an important role in these processes. Fourier transform infrared spectroscopy and gas chromatograph-mass spectrometer analyses of metabolites showed that ring cleavage occurred in the BaP degradation process and the resulting metabolically utilizable substrates were generated as sole carbon sources for B4 growth. Furthermore, mineralization extent of metabolites was verified by determining the total organic carbon and inorganic carbon in the degradation system. |
| Key Words: degradation, mechanism, metabolites, microbial transformation, polycyclic aromatic hydrocarbon |
| Citation: Peng, H., Yin, H., Deng, J., Ye, J. S., Chen, S. N., He, B. Y. and Zhang, N. 2012. Biodegradation of benzo[a]pyrene by Arthrobacter oxydans B4. Pedosphere. 22(4): 554-561. |
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