Pedosphere 19(5): 554--561, 2009
ISSN 1002-0160/CN 32-1315/P
©2009 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Evaluation of biological characteristics of bacteria contributing to biofilm formation |
LI Meng-Ying1,2, ZHANG Ji1, LU Peng1, XU Jing-Liang1 and LI Shun-Peng1 |
1 College of Life Science, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing 210095 (China);
2 College of Life Science, Soochow University, Suzhou 215123 (China) |
| ABSTRACT |
| Biofilm-associated microorganisms play crucial roles in terrestrial and aquatic nutrient cycling and in the biodegradation of environmental pollutants.Biofilm formation was determined for a total of 18 bacterial isolates obtained from the biofilms of wastewater treatment systems and of little carpolite in soil.Among these isolates,seven showed strong biofilm-forming capacity.The phylogenetic affiliation of the isolates showing high biofilm formation capacity was determined through 16S rDNA sequencing and the isolates were grouped into 7 bacterial species including Pseudomonas sp.,Pseudomonas putida,Aeromonas caviae,Bacillus cereus,Pseudomonas plecoglossicida,Aeromonas hydrophila,and Comamonas testosteroni.The biofilm-forming capacity was closely related with flagella,exopolysaccharide,and extracel-lular protein.According to the coefficient of determination,the relative importance of the five biological characteristics to biofilm formation was,in order from greatest to least,exopolysaccharide > flagella > N-acyl-homoserine lactones (AHLs) signaling molecules > extracellular protein > swarming motility. |
| Key Words: bacterial cell characterization, biofilm-forming capacity, path analysis, phylogenetic affiliation, wastewater treatment systems |
| Citation: Li, M. Y., Zhang, J., Lu, P., Xu, J. L. and Li, S. P. 2009. Evaluation of biological characteristics of bacteria contributing to biofilm formation. Pedosphere. 19(5): 554-561. |
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